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Information × Registration Number 0225U004475, (0125U003135) , R & D reports Title Exploring the potential for extracting biopolymeric components from biofilm-forming endophytic microbial communities associated with Antarctic plants popup.stage_title Дослідження потенціалу виділення біополімерних компонентів біоплівок мікробних угруповань ендофітних бактерій, асоційованих з рослинами Антарктики Head Yunhin Olha S., Кандидат біологічних наук Registration Date 09-12-2025 Organization Kyiv National University of Technologies and Design popup.description1 Exploring the potential for Extracting Biopolymeric Components from Endophytic Bacterial Biofilms Associated with Antarctic Plants popup.description2 The potential use of biofilms of microbial communities of endophytic bacteria associated with Antarctic plants as a source of biopolymer secretion under conditions of cultivation of model microbial communities, as well as their individual components, in a wide temperature range has been investigated. This study characterised the obtained biopolymers and validated the potential for developing a production technology using microorganisms associated with plants that possess unique adaptive properties. Physicochemical evaluation revealed that the extraction method and the type of starting sample are crucial factors in determining the final composition and functional properties of the preparations. Precipitation of plankton exopolysaccharide (EPS) samples with ethanol ensured the production of relatively pure polysaccharides with low protein content. In contrast, extraction from biofilm samples resulted in the co-extraction of a significant amount of structural proteins that form the EPS-protein complex, which significantly affects the increase in viscosity and, likely, the surfactant activity of the samples. LCMS visualization confirmed that carbohydrates form the structural basis of the biofilm, and their presence ensures the structural integrity of the biofilm, which is consistent with high viscosity and other physicochemical properties. We screened microorganisms associated with Antarctic vascular plants for their ability to synthesise polyhydroxyalkanoates. The results revealed that certain isolates possess high potential for producing polymers at low temperatures. Consequently, these bacteria offer significant value as producers of biopolymers for eco-friendly applications in medicine, agriculture, and industry. Product Description popup.authors Iryna M. Voloshyna Olena A. Okhmat Olena V. Moshynets Olha S. Yunhin Tetiana O. Kolesnyk Oleksandr O. Kalinichenko Ihor O. Hretskyi popup.nrat_date 2025-12-09 Close