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0225U004747,
(0121U100417)
,
Development of methodical bases of creation of clones in vitro and complex technology of cultivation of paulownia in open soil
Розроблення методичних основ створення клонів in vitro та комплексної технології вирощування павловнії у відкритому ґрунті
Hontarenko Svitlana M.,
18-12-2025
Institute of Bioenergy Crops and Sugar Beet of National Academy of Agrarian Sciences of Ukraine
To develop of methodical bases of creation of clones in vitro and complex technology of cultivation of paulownia in open soil
A method for obtaining viable paulownia explants for introduction into in vitro culture has been developed. The method includes the initiation of growth and development processes in dormant buds on paulownia wood from technical cuttings, on rhizome segments, and on paulownia plants from pot culture through the application of plant growth regulators. It was established that nutrient media supplemented with 6-BAP (0.3–0.5 mg/L), vitamins B1, B6, C, and PP, as well as amino acids, ensure the production of developmentally uniform clones—3–5 clones per planted explant. It was proven that rhizogenesis in paulownia clones under in vitro conditions proceeds more effectively on media supplemented with α-NAA and IBA than on hormone-free media. The growth regulators α-NAA and IBA ensured rapid initiation and uniform root growth—7–11 roots per clone. The addition of GA enhanced the effect of auxins, increasing the number of roots as well as their length and thickness. Media for the initiation of callus formation and callus morphogenesis in paulownia were developed. For callus induction, MS medium was supplemented with 6-BAP at 0.6 mg/L and GA, vitamins, amino acids, and moisture-retaining agents. In media for callus morphogenesis, the dosage of amino acids was increased. This scheme enabled the production of homogeneous paulownia clones.
Svitlana M. Hontarenko
Iryna Shcherbyna
2025-12-18
Updated: 2025-12-18
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