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Information × Registration Number 0225U004795, (0121U100512) , R & D reports Title Development of a method for obtaining and propagation of digaploid clones of androgenic origin of sugar beets and methodological bases of induced androgenesis of рaulownia popup.stage_title Розроблення методу отримання та розмноження дигаплоїдних клонів андрогенного походження буряків цукрових і методичних основ індукованого андрогенезу павловнії Head Hontarenko Svitlana M., Registration Date 18-12-2025 Organization Institute of Bioenergy Crops and Sugar Beet of National Academy of Agrarian Sciences of Ukraine popup.description1 To develop a method for obtaining and propagation of digaploid clones of androgenic origin of sugar beets and methodological bases of induced androgenesis of рaulownia popup.description2 It was established that the vacuolated microspore stage of sugar beet is the most suitable for the stimulation of androgenesis. Using cytological analysis with light microscopy, it was determined that after cold treatment, the majority of microspores in anthers from closed, medium-sized buds are at the vacuolated microspore stage. The system of chemical and physical stress factors for initiating direct in vitro androgenesis in sugar beet anthers includes the application of low-temperature stress using a refrigerated chamber at 4–8 °C for storing branches with buds from seed plants for 10–20 days, followed by placement on modified MS nutrient media supplemented with amino acids, vitamins, growth regulators, and moisture-retaining agents—betaine and trehalose. For the initiation of embryoidogenesis and the cultivation of embryoids, the best results, with an embryoid formation frequency of 10.5–15.1%, were obtained on media 5-3, 5-3, and 7-4B containing polystimulin and abscisic acid (ABA). Product Description popup.authors Svitlana M. Hontarenko Iryna V. Shcherbyna popup.nrat_date 2025-12-18 Close