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Information × Registration Number 0225U004806, (0124U004368) , R & D reports Title Regulatory systems in essential metabolism of Streptomyces albidoflavus J1074: discovery and biotechnological application popup.stage_title Розробка, тестування і застосування біо-сенсорів для контролю генної експресії Head Ostash Bohdan O., Доктор біологічних наук Registration Date 19-12-2025 Organization Ivan Franko National University of Lviv popup.description1 To uncover novel regulatory systems embedded in primary metabolism of Streptomyces albidoflavus J1074 and apply them to assist and inform metabolic engineering popup.description2 Actinomycete genomes harbors hundreds of genes for useful natural products, whose structure and function remain unknown. The main barrier on the way to their studies is very low or even absent transcription (so called silent or cryptic genes). It is necessary to generate new systems of controlled expression of these genes to bypass natural regulatory mechanisms limiting their expression. An idea of this work is to develop such expression systems on the basis of primary metabolic genes of the well-studied streptomycete S. albidoflavus J1074. As a result three transcriptional regulatory genes have been selected having the salient prospects as a basis of such expression systems. These are genes XNR_5147 (effector molecule is gluconate, XNR_3053 (valine), XNR_0155 (butyrate). Conditions of their use have been worked out. J1074 strains have been constructed where these genes control the expression of reporter GFP, whose levels can be directly determined. The constructed strains and approaches were employed to discover novel derivatives of limocrocin and prenylated amino acids. Product Description popup.authors Bahriichuk Kateryna V. Maria Trach popup.nrat_date 2025-12-19 Close