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Information × Registration Number 0225U005022, (0124U002101) , R & D reports Title Development of the microbial phytosorbent as the basis of complex biotechnology for the purification and restoration of soils damaged by military actions popup.stage_title Конструювання і дослідження ефективності розробленого мікробного фітосорбенту для біоремедіації пошкоджених воєнними діями ґрунтів Head Loboda Mariia I., Доктор філософії Registration Date 24-12-2025 Organization Institute of Microbiology and Virology named after D. K. Zabolotny of the National Academy of Sciences of Ukraine popup.description1 The purpose of the work is to develop a phytosorbent using green fallen leaves waste with metal-resistant and growth-promoting bacterial consortium as the basis of a complex biotechnological approach for the depuration and restoration of soils damaged by military actions with the simultaneous production of energy carriers. popup.description2 With soil ecosystems increasingly degraded, biotechnological bioremediation is vital. This study developed a phytosorbent and a consortium of metal-resistant, phytostimulatory microorganisms to restore war-damaged soils. Microbiological and agrochemical properties were assessed, revealing cobalt as the most toxic heavy metal for microorganisms and iron as the least. Bioactive strains were isolated and identified: Streptomyces lidicus SK4, Streptomyces sp. SK16, Streptomyces sp. Y15, Pseudomonas putida L3, Bacillus velezensis L41, and P. frigoritolerance L4, which are active antagonists of phytopathogens. Their enzymatic and biosynthetic properties contribute to the detoxification of contaminated substrates, improvement of the soil microbiome, and stimulation of plant growth. As a phytosorbent, fallen leaves were used; a protocol was adapted with the addition of components to preserve the granule form, and its agrochemical characteristics were determined. The highest organic matter content was observed in the soil sample with the addition of phytopathogens Fusarium spp. and the phytosorbent, with or without the microbial consortium. Under conditions of heavy metal application to soil (Fe(III)+Cu(II), 50 mg/kg each), the content of these elements was measured after 40 days of lawn grass vegetation. The copper content was highest in the soil with the addition of Fusarium spp. and the microbial phytosorbent (57.3 mg/kg), which is likely related to the metabolic activity of bacteria and fungi, whereas Fe in this variant was the lowest compared to those where it was added exogenously at the beginning of the experiment. During anaerobic fermentation of contaminated phytomass, the highest methane concentration was observed when Poa pratensis L. was grown in soil with the addition of the combined preparation contaminated with Fusarium spp., reaching 50% of the gas phase. Product Description popup.authors Bida Iryna O. Havryliuk Olesia A. popup.nrat_date 2025-12-24 Close