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Information × Registration Number 0225U005106, (0120U105754) , R & D reports Title Development of cell selection technology for oil flax resistance to stress factors popup.stage_title Розроблення технології клітинної селекції льону олійного на стійкість до стресових чинників Head Soroka Anatolii I., Registration Date 25-12-2025 Organization Institute of Oilseed Crops of the National Academy of Agrarian Sciences of Ukraine popup.description1 The aim of this project is to develop a technology for cell selection of oilseed flax for resistance to such stressors as salinity and water deficiency. popup.description2 Oilseed flax is an important agricultural crop grown in Ukraine in regions with varying types and degrees of salinity. Cell selection techniques enable the generation of genotypes adapted to saline soils by selecting cells in vitro under selective pressure. We have for the first time developed a cell-selection technology for salt chloride tolerance, which includes material preparation, induction of callus formation, concentration and duration of exposure to the selective agent, induction of organogenesis, rooting of shoots, and initial adaptation to natural conditions. Both N6 and MS media supplemented with 2.0 mg/L 2,4-D promoted well-developed callus formation. Sodium chloride at concentrations of 0.5–1.5% inhibited callus cell growth and, at the highest concentrations, caused their death. NaCl at concentrations of 0.3–0.6% ensured effective selective screening while maintaining sufficient regenerative potential. Cytokinins zeatin, benzyladenine, and thidiazuron at 1.0 mg/L were effective for shoot regeneration. Methods for direct and indirect exposure of flax callus to a mutagenic agent were developed. In direct treatment, ethyl methanesulfonate was effective at 0.2% for 2 hours, and nitrosomethylurea at 0.2% for 1–2 hours. In indirect treatment, optimal cultivation of callus on media containing ethyl methanesulfonate ranged from 0.1–0.05%, and for nitrosomethylurea — below 0.01%. For rooting regenerated shoots, application of auxins — naphthaleneacetic acid or indole-3-acetic acid at 1 mg/L — was optimal, with the former being more effective. It has been established that the proportion of flax regenerants obtained after selection in callus-culture that are resistant to elevated NaCl concentration across different genotypes ranged from 7–9%. The developed technology of cell selection for salinity tolerance in oilseed flax can be used for the accelerated development of plant material adapted to various stress factors. Product Description popup.authors Anatoly I. Soroka popup.nrat_date 2025-12-25 Close