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Information × Registration Number 0225U005131, (0124U002066) , R & D reports Title Development of new hybridization DNA sensors modified by nanomaterials based on surface plasmon resonance popup.stage_title Оптимізація функціонування та дослідження аналітичних характеристик нової модифікованої НЧЗ біосенсорної системи для визначення 80-mer BCR-ABL. Порівняння отриманих параметрів з характеристиками традиційних ДНК-біосенсорів. Head Kucherenko Ivan S., Кандидат біологічних наук Registration Date 25-12-2025 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description1 The aim of the work is to develop a new technology for using nanomaterials to create DNA biosensors based on PPR with improved analytical characteristics. popup.description2 As part of the second stage of the project, testing of the proposed new biosensor system based on PPR modified with gold nanoparticles and a measurement method based on the "sandwich analysis" was carried out. Also, within the framework of the project, a universal procedure for measuring the concentration of 80-mer BCRABL in liquids using the developed biosensor system was proposed and described. During the study, optimal measurement conditions were selected using the proposed biosensor system, and a method for regenerating the developed biosensor system for multiple use was also described. A new method for chemical synthesis of gold nanoparticles was proposed, which makes it possible to improve the selectivity and stability of biosensor analysis. Based on the results of the analysis of the analytical characteristics of the system, a multiparameter comparison of the new modified NNP biosensor system with a traditional DNA biosensor was carried out. Using the example of the proposed biosensor system for the determination of CML, the prospect of using gold nanoparticles to improve SPR signals and, accordingly, improve the analytical characteristics of DNA biosensors based on SPR has been proven. Product Description popup.authors Kucherenko Daria Yu. Daryna O. Mruha Maksym S. Sobolevskyi popup.nrat_date 2025-12-25 Close