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Information × Registration Number 0225U005162, (0123U100036) , R & D reports Title Estimation of the level of extracellular vesicle / exosome production by human mesenchymal stromal / stem cells from various tissue sources popup.stage_title Оцінка рівня продукції позаклітинних везикул/екзосом мезенхімальними стромальними/стовбуровими клітинами людини з різних тканинних джерел Head Novikova Svitlana M., Доктор медичних наук Registration Date 26-12-2025 Organization State Institution "National Scientific Center "Institute of Cardiology, clinical and regenerative medicine named after academician M.D. Strazhesko" of the National Academy of Medical Sciences of Ukraine popup.description1 Evaluation of the level of production of extracellular vesicles/exosomes by human mesenchymal stromal/stem cells from different tissue sources for the purposes of regenerative medicine, use in pharmacological research and in clinical pharmaceutical production popup.description2 Object – extracellular vesicles/exosomes from human producer cells from various tissue sources. Research goal: assessment of the level of extracellular vesicles/exosomes production by human mesenchymal stromal/stem cells from various tissue sources for the purposes of regenerative medicine, use in pharmacological research and in clinical pharmaceutical production. Methods: cultural, cytochemical, colorimetric, immunoenzymatic, flow cytometry, NTA-method, statistical. Main results: within the framework of the research, populations of PV-producing cells of adipose tissue MSCs (VSC-MSCs), umbilical cord MSCs (UCMSCs) and neural crest-derived MSCs from dental pulp (NCMSCs) were obtained, which meet all the necessary criteria for determining human MSCs, namely: classical morphology, ability to form colonies, inherent immunophenotype, ability to direct differentiation in adipogenic and osteogenic directions. PVs were isolated and characterized from the conditioned medium of cells-producing VSCs, VSCs and UCMS. PVs were studied for distribution in concentration and size. The presence of specific markers of small PVs – CD63 and CD81 – was established and the protein level was determined. Thus, the production of vesicles that correspond to small PVs in their characteristics was confirmed. It was found that MSC-VT produce PV on average 300 ml/ml less compared to MSC-PC and MSC-PNG. It was also noted that the concentration of PV is 2-3 times higher when cultivating MSCs of all three types in MEM medium without L-glutamine and phenol compared to Hanks. In general, it was found that all types of MSC produce PV in sufficient quantity - 1 billion particles per ml, which, according to regulatory documents, is sufficient for use in medical and clinical purposes. Product Description popup.authors Злацький Ігор Анатолійович Зубов Дмитро Олександрович Alona V. Zlatska Marina Shamshur popup.nrat_date 2025-12-26 Close