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Information × Registration Number 0225U005187, (0125U000950) , R & D reports Title Development of technology for creating biosensor test systems for analyzing enzyme activity as diagnostic indicators of internal organ damage popup.stage_title Розробка біосенсорних методик вимірювання активності ферментів групи гідролаз, кіназ та трансфераз. Розробка амперометричних біосенсорів для визначення активності КК, альдолази, ЛДГ та АСТ. Оптимізація конструкції та умов функціонування розроблених біосенсорів. Дослідження основних аналітичних характеристик ензим-чутливих біосенсорів, вивчення їх селективності та стабільності. Head Dziadevych Serhii V., Доктор біологічних наук Registration Date 26-12-2025 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description1 Development of the novel technology for creating biosensor test systems for analyzing enzyme activity in biological fluids for the purpose of early diagnosis of damage to human internal organs popup.description2 Several amperometric biosensors have been developed to determine the levels of the most important enzyme diagnostic markers in biological fluids. In particular, a biosensor was developed to determine creatine kinase (CK) based on the competition of immobilized hexokinase for ATP of the target CK in the analyzed sample. Two more biosensors developed in the work are intended for determining the activity of transferases (AST and ALT). Detection of the activity of these enzymes occurs by determining the concentration of their products (glutamate and pyruvate, respectively), which within certain limits is directly proportional to the activity of a particular transferase. Several biosensor principles for determining aldolase activity have been developed and the principle possibility of creating such devices has been proven. The analysis was based on the use of multi-enzyme cascades of immobilized enzymes and their competition for aldolase substrates in solution. An amperometric biosensor was developed to determine the activity of lactate dehydrogenase (LDH) in biological fluids. In order to form bioselective membranes of amperometric biosensors, specific immobilized enzymes were used that interact with target enzymes (CC, LDH, AST, ALT, aldolase) in the analyzed solution. Several immobilization options were analyzed and optimal methods were selected for creating the corresponding biosensors. Optimal conditions for the immobilization process were selected, namely the duration of immobilization, the concentration of the enzyme and crosslinking agent, etc. Also, for all developed biosensors, optimal concentrations of substrates and coenzymes were selected that must be added to the analyzed solution during analysis. The influence of the main solution parameters on the functioning of these biosensors, such as pH, ionic strength, buffer capacity, protein concentration, temperature, was studied. The selectivity of the work of all proposed biosensors was studied. Product Description popup.authors Liudmyla V. Shkotova Oleksandr O. Soldatkin Korpan Yaroslav I. Gorodnia Oleksandra V Daryna O. Mruha Tetyana A. Sergeyeva Maksym S. Sobolevskyi Berketa Kseniia O. Bakhmat Veronika A. Biloivan Olha A. Valentyna M. Arkhypova Yevhen Vakhovskyi Angelina Buzhak Iryna Knyr Yulia Panasyuk popup.nrat_date 2025-12-26 Close