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Information × Registration Number 0226U000917, (0124U000993) , R & D reports Title Obtaining by biotechnological methods initial and linear material of wheat, adapted to zonal agro-climatic conditions popup.stage_title Отримання біотехнологічними методами вихідного та лінійного матеріалу пшениці, адаптованого до зональних агрокліматичних умов Head Zambriborshch Iryna S., Кандидат біологічних наук Registration Date 19-01-2026 Organization Plant Breeding and Genetics Institute - National Center of Seed and Cultivar Investigation popup.description1 Obtaining wheat initial material using in vitro biotechnological methods popup.description2 During the reporting year, a comprehensive set of studies was carried out to improve biotechnological methods for generating genetically stable initial material of winter bread wheat. The research also aimed to increase the efficiency of using doubled haploid (DH) lines in breeding programs. The sterilization conditions of the explant material were optimized, as well as the composition (including the iron source) and the type of nutrient media. It was established that shortening the sterilization time improves microspore viability. The W14 medium was identified as the most productive among all tested variants for androgenesis induction. In total, 76 DH wheat lines were obtained and characterized. Among them, 52 lines showed complex resistance to six of the most widespread wheat diseases. Molecular-genetic analysis enabled the identification of key resistance genes, including Lr26/Sr31/Pm8/Yr9, Lr34/Yr18/Sr57/Pm38, Lr68, Sr58, Pm39, and Yr29. These genes are associated with polygenic, race-non-specific resistance to rust diseases and powdery mildew. Four DH lines (No. 115 DH1, No. 207 DH1, КП186 DH1, and КП230 DH2) were found to carry three or more resistance-gene loci (Lr34+Lr46+Lr68, Sr31+Sr58, Pm38+Pm39, Yr18+Yr29, Bdv1). This makes them valuable donors for future breeding programs. Based on a portion of the DH lines obtained earlier, a new winter bread wheat cultivar, Moment, was jointly developed by specialists from three departments. The cultivar demonstrates group resistance to major pathogens and has been submitted for State Variety Testing. This achievement confirms the high practical value of the obtained results. It also demonstrates the effectiveness of combining in vitro androgenesis with marker-assisted selection in modern breeding, in line with leading international practices. Product Description popup.authors Mykola A. Lytvynenko Oleksii A. Vasyliev Oksana L. Shestopal Mariya S. Chekalova Olexandr A. Afinogenov Oleksiy V. Halaev Mariya V. Halaeva popup.nrat_date 2026-01-19 Close