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Information × Registration Number 0226U001076, (0123U101790) , R & D reports Title The search for markers of post-traumatic stress disorder based on indicators of oxidative stress and inflammation popup.stage_title Пошук маркерів посттравматичного стресового розладу на основі показників оксидативного стресу та запалення Head Bailiak Mariia M, Доктор біологічних наук Registration Date 21-01-2026 Organization Vasyl Stefanyk Precarpathian National University popup.description1 To develop markers for the clinical diagnosis of PTSD based on indicators of oxidative stress and inflammation as an addition to the existing arsenal of psychological methods popup.description2 Within the framework of the project, a comprehensive study of the behavioral, biochemical, immune, and metabolic mechanisms of post-traumatic stress disorder (PTSD) was conducted using validated mouse models. Molecular and biological approaches allowed PTSD to be characterized as a multisystem disorder with long-term consequences. Different stressors, including social isolation, prolonged immobilization, simulation of predator presence, and electric shock trauma, were shown to induce stable anxiety-like and cognitive impairments specific to PTSD. Age- and sex-related differences in stress sensitivity were identified. Analysis of blood parameters and brain tissues demonstrated that PTSD is accompanied by activation of the immune response and oxidative stress. In stressed animals, hyperglycemia, leukocytosis, and an imbalance between pro- and antioxidant systems were observed. In the cerebral cortex, increased lipid peroxidation products and decreased activity of glutathione system enzymes were detected, indicating depletion of antioxidant potential. An important outcome of the project was confirmation of the feasibility of using paraoxonase (PON) as a component of a multifactorial panel of biochemical markers of PTSD. Optimization of the method for determining arylesterase activity of PON ensured high sensitivity and reproducibility, and its practical value was confirmed by submission of a patent application (u202405523). The combination of immune activation and stress exposure enhances anxiety-like behavior, oxidative stress, and molecular disturbances in the brain, including changes in gene expression related to cytokine and glucocorticoid signaling and activation of microglia. Social isolation combined with a high-calorie diet increases the risk of metabolic disorders. Overall, the project results have high scientific and translational significance and provide an experimental basis for the development of biomarker-based approaches to the diagnosis of PTSD. Product Description popup.authors Volodymyr I. Lushchak Oleksandra B. Abrat Lushchak Oleh V. Dmytro V. Hospodarov Halyna V. Shmihel Vladyslav V. Berezovskyi Mariia P. Lylyk Olha M. Strilbytska Oleh I. Demianchuk Mariia M. Bailiak Shvadchak Volodymyr V. Vitalii A. Balatskyi popup.nrat_date 2026-01-21 Close