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Information × Registration Number 0226U001334, (0125U000980) , R & D reports Title Biotechnological plants for combating infectious bacterial diseases and phytodestruction of military-origin pollutants popup.stage_title Створення генетичних конструкцій з генами сальмоцину та цитохромів,перевірка їх функціональності та отримання відповідних трансгенних рослин Head Kuchuk Mykola V., д.б.н. Registration Date 23-01-2026 Organization Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine popup.description1 Creation of transgenic forage crops (alfalfa, maize, etc.) that accumulate salmocin (a bacteriocin protein that selectively kills Salmonella) and exhibit antibacterial activity, as well as the development of transgenic green manure plant lines (oilseed radish, alfalfa, etc.) resistant to herbicides and capable of enhanced phytodestruction and soil remediation from military-origin organic pollutants. popup.description2 Subject of study: biotechnological plants synthesizing recombinant antibacterial proteins, and transgenic plant lines capable of enhanced phytodestruction and remediation of soils contaminated with organic and inorganic substances of military origin. The objective of the scientific research is: to develop transgenic forage crops (alfalfa, maize, etc.) that accumulate salmocin (salmocin - bacteriocin that selectively kills Salmonella) and exhibit antibacterial activity, as well as to develop transgenic plant lines capable of enhanced phytodestruction and soil purification from organic substances of military origin. During the reporting period, genetic vectors were constructed, and transgenic plants containing genes encoding salmocin - an antibacterial protein that neutralizes Salmonella - were obtained. To produce recombinant salmocin in plants, both ethanol-inducible and constitutive expression systems were utilized. In both cases, the antimicrobial effect of the biotechnological plant extracts was confirmed against Salmonella strains. Experiments conducted with transgenic plants producing recombinant colicin M confirmed that it maintains its antibacterial activity even after four years of storage. The addition of 20 kg of such dry plant biomass per ton of mixed fodder results in the formation of pellets that also demonstrate antibacterial activity by inhibiting the growth of Escherichia coli. A screening of agricultural plants for resistance to high concentrations of heavy metals and polycyclic aromatic hydrocarbons (PAHs) was performed, revealing that several species (specifically, rapeseed) demonstrate significant tolerance and can serve as potential phytoremediators. Genetic vectors were constructed with genes encoding enzymes (XplA/XplB, bacterial laccase, human cytochrome P450) suitable for the phytodestruction of explosive residues and PAHs. Product Description popup.authors Andrii Potrohov Borysiuk Mykola V. Nadiia A. Matvieieva Овчаренко Ольга Олександрівна Булко Ольга Володимирівна Кучук М.В. Щербак Наталія Леонідівна Листван Катерина Володимирівна Сіндаровська Я.Р. Рудас Володимир Андрійович Белокурова Валерія Борисівна Loshyna Liudmyla H. Taisa A. Bohdanovych popup.nrat_date 2026-01-23 Close