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Information × Registration Number 0226U001512, (0124U001408) , R & D reports Title To optimize the technology of obtaining embryos of cattle and pigs using frozen-thawed gametes popup.stage_title Оптимізувати технологію одержання ембріонів великої рогатої худоби та свиней із використанням деконсервованих гамет Head Shcherbak Oksana V., кандидат с.-г. наук, старший науковий співробітник Registration Date 27-01-2026 Organization Institute of Animal Breeding and Genetics nd. a. M.V.Zubets of National Academy of Agrarian Science of Ukraine popup.description1 Based on the application of different time parameters of the start of activation of in vitro maturation of oocytes, frozen-thawed oocytes of pigs, optimize the technology of obtaining in vitro embryos of cattle and pigs. popup.description2 According to the results of research in 2024–2025, the influence of the time parameters of the onset of oocyte maturation and the choice of culture medium for obtaining embryos in vitro was established. For cattle, the optimal maturation time is up to 6 hours, which ensures a high level of maturation and crushing of embryos. For pigs, the use of native oocytes demonstrates stable development in two environments, with a tendency to higher indicators in "CDM", while for depreserved oocytes, "Sage 1-Step" is more effective. In general, the efficiency of embryo development from depreserved gametes remains lower, which emphasizes the need for further optimization of cryopreservation and in vitro cultivation methods. Product Description popup.authors Oksana V. Shcherbak Svitlana I. Kovtun Petro A. Trotskyi Volodymyr F. Stakhovskyi Anatolii O. Meleshko Oksana Y. Lyzohub Nataliia V. Shykova Volodymyr V. Chernenko popup.nrat_date 2026-01-27 Close
R & D report
Head: Shcherbak Oksana V.. To optimize the technology of obtaining embryos of cattle and pigs using frozen-thawed gametes. (popup.stage: Оптимізувати технологію одержання ембріонів великої рогатої худоби та свиней із використанням деконсервованих гамет). Institute of Animal Breeding and Genetics nd. a. M.V.Zubets of National Academy of Agrarian Science of Ukraine. № 0226U001512
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Updated: 2026-01-28