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Information × Registration Number 0226U001840, (0123U102054) , R & D reports Title Designing diagnostic tools for pathogens of current endemic zoonoses with high genetic and phenotypic potential for pathogenicity popup.stage_title Конструювання засобів діагностики збудників актуальних ендемічних зоонозів з високим генетичним та фенотиповим потенціалом патогенності Head Vyhovska Liliia M., Доктор ветеринарних наук Registration Date 02-02-2026 Organization National University of Life and Environmental Sciences of Ukraine popup.description1 The purpose of the work is to create diagnostic tools for emerging endemic zoonoses using genetic and phenotypic factors of pathogenicity popup.description2 The work used microbiological methods (bacteriological studies of biological material on nutrient media, determination of the nature of vegetation, colony morphology - size, shape, consistency, surface character, pigmentation, etc., preparation of smears, their staining and microscopy, study of enzymatic properties), serological (RA), molecular genetic (identification and typing of isolates of microorganisms in PCR), mathematical and statistical (processing of the obtained data using the Microsoft Excel 2007 application package). A study of pathological and biological material from animals, animal feed for microbiological indicators was carried out; isolates of pathogens were isolated and their identification was carried out, signs of pathogenicity were determined - virulence, resistance to antimicrobial drugs, ability to form biofilms. Potential risks of formation of foci of resistant pathogens in poultry (chickens, ducks, quails, pheasants) kept on household plots were determined. Indicator microorganisms isolated from poultry were studied regarding their ability to form biofilms. Based on the results of the research, a laboratory sample of a tool for diagnosing zoonotic pathogens and detecting signs of pathogenicity in them was developed and manufactured - a set of strains for testing chromogenic media and its effectiveness was assessed. Technological regulations for a tool for diagnosing zoonotic pathogens and detecting signs of pathogenicity in them were prepared. Scientific and methodological recommendations were prepared regarding the procedure for using the developed tools A study was conducted to determine the sequence of fragments of the 16S rRNA gene of the isolated isolates, data on the strains were entered into the National Center for Biotechnology Information. In addition, the prospects for the use of feed additives containing probiotic strains and nanomaterials were investigated with regard to the possibility of their use to increase the resistance Product Description popup.authors Tetiana V. Mazur Serhii I. Holopura Machuskyi Oleksandr V Mykola L. Radzykhovskyi Liliia M. Vyhovska Valerii O. Ushkalov Oleksandr H. Martyniuk Volodymyr V. Melnyk Viktor M. Shevchuk Mykola F. Starodub Nataliya H. Sorokina Serhii O. Boianovskyi Bezpalko Oleksandr O. Liliana O. Davydovska Viktor М. Lytvynenko Yurii Y. Vishovan Vitalii V. Nedosekov Artem V. Ushkalov Tetiana S. Markovska popup.nrat_date 2026-02-02 Close