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Information × Registration Number 0226U001949, (0125U002918) , R & D reports Title The role of meprins in modulating the stromal microenvironment of prostate cancer popup.stage_title Аналіз особливостей експресії МEР1A та МEР1B у тканині РПЗ та ідентифікація їх зв’язку із особливостями стромального мікрооточення новоутворень in silico. Дослідження особливостей експресії мепринів на рівні мРНК у пухлинній тканині хворих на РПЗ із різним метастатичним статусом. Head Zadvornyi Taras V., Кандидат біологічних наук Registration Date 03-02-2026 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology National Academy of Sciences of Ukraine popup.description1 To identify the features of the stromal microenvironment of prostate cancer with different metastatic potentials associated with altered expression of meprins (MEP1A and MEP1B) popup.description2 In an ex vivo system, it was demonstrated that prostate cancer (PCa) tissue is characterized by 2.6-fold (p<0.05) and 1.9-fold (p<0.05) higher mRNA levels of MEP1A and MEP1B, respectively, compared with benign prostatic hyperplasia tissue. An association was identified between MEP1A mRNA levels and the T (p<0.05) and M (p<0.05) categories according to the TNM classification, as well as the Gleason score (p<0.05). No association was found between MEP1A mRNA levels and metastatic involvement of lymph nodes or the preoperative serum PSA level. It was shown that the level of MEP1B mRNA in PCa tissue from patients with distant metastases (M1 category according to the TNM classification) was 2.2-fold (p<0.05) lower compared with the corresponding value in patients without distant metastases. No associations were demonstrated between MEP1B mRNA levels and the main clinicopathological characteristics of PCa, including the T and N categories according to the TNM classification, the Gleason score, or the preoperative serum PSA level. Analysis of meprin expression in PCa tissue from patients with different metastatic status revealed that MEP1A and MEP1B mRNA levels were 1.5-fold (p<0.05) and 2.2-fold (p<0.05) lower, respectively, in patients with distant metastases (to bone and visceral organs) compared with patients with metastases limited to regional lymph nodes. Based on bioinformatic analysis using the TIMER 3.0 database, correlations were identified between MEP1A expression and infiltration levels of M1- (ρ=0.36) and M2- (ρ=0.25) polarized macrophages, as well as tumor-associated fibroblasts (ρ=0.19). MEP1B levels correlated with infiltration levels of CD4⁺ (ρ=0.19) and CD8⁺ (ρ=0.17) T lymphocytes, as well as tumor-associated fibroblasts (ρ=0.17). Product Description popup.authors Oleksandr M. Mushii Tetiana S. Burda popup.nrat_date 2026-02-03 Close