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Information × Registration Number 0226U002148, (0125U000855) , R & D reports Title Biotechnologies for increasing stress resistance of plant systems and their use for phytoremediation popup.stage_title Скринінг різних видів рослин на їх здатність до фіторемедіації. Створення генетичних векторів з генами, експресія яких підвищує стресостійкість рослин та/або їх фіторемедіаційні властивості. Head Kuchuk Mykola V., д.б.н. Registration Date 09-02-2026 Organization Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine popup.description1 Screening of various plant species for their ability to phytoremediation; creation of biotechnological crops with improved phytoremediation properties and increased stress resistance by genetic engineering methods; identification of the most promising biotechnological plant lines for phytoremediation and restoration of contaminated areas popup.description2 Object:, biotechnological plants plants and in vitro cultures Purpose: to investigate and substantiate the possibilities of applying modern biotechnological approaches to enhance the stress tolerance of plant systems and strengthen their phytoremediation potential through screening of various plant species for phytoremediation capacity, as well as through the use of genetic vectors carrying genes whose expression contributes to increased plant resistance to abiotic stresses and/or improves the efficiency of pollutant removal from the environment. The study examines integrated approaches to improving plant resistance to abiotic and biotic stress factors using physiological, molecular genetic, and biotechnological methods. Endemic plant species were screened to identify effective phytoremediators capable of absorbing and accumulating heavy metals, in particular copper and zinc. The effects of CML30 and WAK280 gene knockouts on pathogen resistance in different potato cultivars were determined. An efficient method for genetic transformation of potato was developed, and an in vitro collection of transgenic lines carrying extracellular RNase genes (ZRNase, bov) that confer enhanced resistance to viral infections was created. The role of the proteins EXECUTER1 and EXECUTER2 as key messengers of singlet oxygen–induced programmed cell death was analyzed. Using the model plant Nicotiana benthamiana, an interaction between viral infection and lead contamination was demonstrated, and a limiting effect of lead on the spread of tobacco mosaic virus was established. In addition, the effectiveness of using in vitro culture of immature sunflower embryos to accelerate homozygotization of lines resistant to the herbicide tribenuron-methyl was demonstrated. The obtained results have practical significance for plant biotechnology, breeding, and environmental plant biology. Product Description popup.authors Lystvan Kateryna V. Ovcharenko Olha O. Symonenko Yurii V. Potrokhov Andrii O. Kyrpa Tetiana M. Loshyna Liudmyla H. popup.nrat_date 2026-02-09 Close