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Information × Registration Number 0226U002266, (0121U110055) , R & D reports Title Determination of molecular processes involved in the development of preeclampsia through the integration of transcriptome, proteome and metabolic data in the human placenta popup.stage_title Інтеграція даних транскриптому, протеому та метаболому, отриманих високопродуктивними методами молекулярної біології в плаценті людини за умов фізіологічного перебігу вагітності і прееклампсії Head Obolenska Mariia Yu., д.б.н. Registration Date 12-02-2026 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description1 Preeclampsia is a life-threatening disease of pregnant women, which occurs with a frequency of 5-10% in the world and is characterized by high blood pressure and the appearance of protein in the urine after the 20th week of pregnancy. In case of untimely termination of pregnancy, preeclampsia turns into eclampsia - convulsions, which often end in the death of mother and child. Placental dysfunction plays a leading role in the occurrence of preeclampsia. The aim of the project is to conduct a systematic analysis of the transcriptome, proteome and metabolite in the human placenta under physiological conditions of pregnancy, preeclampsia and in women smokers, in whom the incidence of preeclampsia is twice lower than in women who do not smoke. Identifying the factors that contribute to and support the disease and that prevent its occurrence is one of the features of this project. To achieve the main goal of the project, two approaches will be used - bioinformatics analysis of publicly available data and experimental studies aimed at validating the results of bioinformatics analysis, search for new markers of the pathological process and factors that contribute to seizures. popup.description2 Comparative analysis of gene expression in the placenta during the first and second halves of pregnancy (comparison of intervals 1–2 and 2–3) identified 54 shared differentially expressed genes. Based on the dynamics of expression changes, these genes were classified into four categories: UpUp and DownDown, characterized by a monotonic increase or decrease in expression, respectively, and UpDown and DownUp, in which the direction of expression changes shifts during the transition from the second to the third trimester. In the first half of pregnancy, the most pronounced changes within the UpUp group were observed for the genes SERPINB2, EBI3, GH1, C7, and CSHL1, whereas in the second half of pregnancy the most significant changes involved MAGEA10, SEMA3B, PAPPA, and CSH1. Within the UpDown group, 11 of the 21 genes are associated with pro-inflammatory activity and increased chemokine expression. The remaining ten genes in this group are involved in the regulation of apoptotic processes, affect vascular tone, and promote tissue growth. Genes in the DownDown group participate in the early stages of placental development and blastocyst implantation (PAEP, AFP, HBE1, IRX3, and OSR2). A distinct functional subgroup includes LOX, FLRT2, and AGR2, whose expression is associated with extracellular matrix function and intercellular interactions. These genes contribute to reduced matrix density, enhanced cell migration, and angiogenesis. In particular, LOX (lysyl oxidase) encodes a Cu²⁺-dependent enzyme that catalyzes the cross-linking of collagen and elastin, provides mechanical stability to the functionally mature extracellular matrix, and influences cell migration, angiogenesis, and cell–cell interactions. FLRT2 regulates intercellular contacts in developing tissues, whereas AGR2 may promote epithelial–mesenchymal transition (EMT), thereby facilitating cell migration. Product Description popup.authors Frolova Alina O. Martseniuk Olha P. Chabanova Mariia V. Rodriguez Ruslan Reynaldovych Oleksandr . K. Lykhenko popup.nrat_date 2026-02-12 Close