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Information × Registration Number 0226U002978, (0123U100462) , R & D reports Title Study of vital activity peculiarities of biotechnological plants after genomic modifications popup.stage_title Генетична трансформація пшениці створеними векторами; вивчення експресії трансгенів та адаптивності змінених рослин Head Morhun Bohdan V., Доктор біологічних наук Registration Date 11-03-2026 Organization Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine popup.description1 The purpose of scientific research is to study the possibility of genetic modification of economically useful cultivated plants with heterologous genes of various origins and to find out the peculiarities of their inheritance, phenotypic manifestation of signs, physiological and biochemical properties of bioengineered plants in comparison with the original forms. popup.description2 For the first time in the world, spelt somaclones were obtained using immature embryo culture, and significant somaclonal variability was detected in terms of structural and morphological parameters, grain size, total soluble protein content, and photosynthetic pigments in spelt regenerants as a result of intragenomic rearrangements caused by the movement of mobile genetic elements. It was established that a greater number of productive spelt somaclones can be obtained on regeneration media with a high content of 6-benzylaminopurine (BAP), regardless of the medium used for callus induction. A comparison was conducted of the morphological features of the stomatal apparatus in spherical-grain wheat, Isfahan wheat, Sinai wheat, and spelt. The effect of short-term drought on bread wheat, spherical-grain wheat, and spelt was studied. It was found that in bread wheat of the Podolyanka variety, short-term drought leads to a significant decrease in total plant mass and grain mass, while in spelt (Triticum spelta var. duhamelianum) it causes a significant decrease in the number of spikelets and the harvest index. Spherical-grain wheat (Triticum sphaerococcum var. rubiginozum L 322-15) proved to be the most sensitive to short-term drought, showing a significant reduction in the number of spikelets, grain weight, number of grains, and harvest index. Among the five tested genotypes of bread wheat, the highest frequency of regeneration from callus of apical origin was observed in the varieties Zymoyarka and Podolyanka, while a medium regeneration frequency was characteristic of the variety Yatran 60. Accordingly, these bread wheat genotypes are suitable for Agrobacterium-mediated transformation in in vitro culture. Product Description popup.authors Iryna O. Nitovska Olha V. Pronina Марія О. Банникова popup.nrat_date 2026-03-11 Close