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Information × Registration Number 0299U000571, 0193U034441 , R & D reports Title The influence of impairmed regulation of enzymes of flavinogenesis biosynthesis and purine metabolism on riboflavin productivity in yeast popup.stage_title Head Fedorovych D.V., Registration Date 27-04-1999 Organization Division of Regulatory Cell Systems Institute of Biochemistry popup.description2 It was revealed that unification of mutation of negative action type (rib80,rib81 and hit) in one genome causes the increase in flavinogenic activity in yeast Pichia guilliermondii. It was shown that enlarged supplement by purines and increase in pentose contents in the yeast cells caused the increase in flavinogenic activity in wild type strains and in mutants with impaired regulation of flavinogenesis. The plasmids were constructed that contained RIB1 gene of P.guilliermondii or ribA gene of Bacillus subtilis under the cotrol of this yeast promoter or under the control of TEF - promoter of Saccharomyces cerevisiae. It was demonstrated that the replacement of RIB1-promoter by TEF - promoter led to increase in level of flavinogenesis and GTP-cyclohydrolase activity. The introduction of ribA gene increased insufficiently the flavinogenic activity of P.guilliermondii. It was constructed recombinant plasmids that contained RIB7gene of P.guilliermondii as a part of fragments of DNA of different sizes. It was shown that the level of this gene expression did not depend on the size of insert. The shuttle-vector P.guilliermondii/Escherichia coli that provided the high efficiency of transformation was constructed. It was constructed yeast strains that synthesized up to 1 g/l of vitamin B2 while growth on media which contained distillery refuse and molasses. These strains can be used in biotechnology of feed that contains vitamin B2. Product Description popup.authors popup.nrat_date 2020-04-03 Close