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Information × Registration Number 0305U005759, 0104U003210 , R & D reports Title To develop of technologies for obtaining of dendritic cells and reveal of possibilities for their use in creation of antitumor vaccines of new generation popup.stage_title Визначити найбільш ефективні конструкції протипухлинних вакцин на основі дендритних клітин шляхом дослідження їх протипухлинної активності в експерименті Head Grinevich Y.A., Registration Date 23-12-2005 Organization Ukrainian Research Institute of Oncology and Radiology popup.description2 The aim of the study – To develop of tecknologies of autologous antitumor vaccines with using of dendritic cells and reveal of possibilities for their use for improvement of cancer patients treatment efficacy. Technological regimen for obtaining of dendritic cells from peripheral blood leukapheresis monocytes in the patients with cancer have been developed. Using phenotyping, functional tests and cytomorphological examinations it has been shown, that maturity of obtained DC depend on concentration of tumor antigen and route of their administration. Realization of antitumor and antimetastatic effect of DC-vaccines is at the expense of stimulating influence on lymphoid tissue of recipient. DC administration results in increase of amount of lymphoblasts in thymus, immunoblasts, plasmacytoid cells, CD3+,CD4+,CD8+ - lymphocytes in spleen, absolute amount of lymphocytes, CD8+ - lymphocytes and large granular lymphocytes in peripheral blood, as well as essential increase of cytotoxic activity of splenocytes. Insrease of CD4+/CD8+- lymphocytes correlation in spleen and decrease of functional activity of DC in vitro in mixed leukocyte reaction with allogeneic lymphocytes is followed by worse antimetastatic efficiency of DC, loaded with lyophilized tumor cells (LTC) or mechanomodification (m/m) LTC. Product Description popup.authors popup.nrat_date 2020-04-02 Close