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Information × Registration Number 0306U001285, 0103U001409 , R & D reports Title The comparative characteristic of features cd34 + cells of a various parentage for the further substantiation of expediency of their use at auto- and allotransplantations. popup.stage_title Вивчити та провести порівняльний аналіз цитокінового фону та рецепторної активності субпопуляцій на цитокінову стимуляцію гемопоетичних клітин в культурі кісткового мозку, пуповинної, периферичної крові та ембріональної печінки та вивчити морфофункціональні властивості СД 34+ клітин в залежності від термінів зберігання. Head Бебешко Владимир Григорьевич, Registration Date 20-02-2006 Organization Research Centre for Radiation Medicine popup.description2 Object of research mononucleares an bone marrow, cord and a peripheric blood and a embrional liver. The purpose of work to learn and carry out the comparative analysis of cytokine background and receptor activity of subpopulations on cytokine stimulation of hemopoietic cells in culture of an bone marrow, cord, peripheric blood and embrional liver and to learn morphofunctional properties CD34 + cells on storage periods. Methods of research - morphological, immunological, cytochemical, immunofermentic and cultural. The cytokine background and receptor activity of subpopulations on a cytokine stimulation of hemopoietic cells of a different parentage was determined. It fixed, that a level of GM - CSF, SCF and receptor SCF (CD - 117) in supernatantesof cultures of peripheric blood cells of healthy donors made accordingly-3,08+0,5 pg/ml, 11,1+3,1pg/ml and 1,09+0,85 pg/m,cord bloods - 3,73+0,8 pg/m, 19,1+4,6 pg/m and 2,48+0,2 pg/m, a embrional liver-3,5+0,9 pg/m, 17,4+3,5 pg/m and 2,02+0,2 pg/ml.The maximal concentration GM-CSF and SCF in supernatante, the different parentage received from culture SC, were determined after 24 hours of culturation. In 72 hours the level GM - СSF and SCF was reduced, for 7 day - was the lowest. The high level of SCF receptor was determined in stimulation PHA culture after 72 hours of cultivation. Long-term storage of stem cells received from different sources, in conditions of low temperatures essentially does not change ability to different lineas to differentiation and proliferative activity of cells - precursors, and also their phenotype.5635 Product Description popup.authors popup.nrat_date 2020-04-02 Close