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Information × Registration Number 0307U003076, 0106U002355 , R & D reports Title Investigation of ionizing radiation impact on animal sexual cells popup.stage_title Дослідити вплив різних доз та режимів опромінення ізольованих гамет на їх функціональні властивості та репродуктивні характеристики Head Аndrejchenko S.V.; Таlko V.V., Registration Date 17-01-2007 Organization Research Centre for Radiation Medicine popup.description2 Object - functional and reproductive characteristics of male and female gametes from experimental animals under X- and gamma-irradiation in various doses and different regimens. Methods - radiobiological, biochemical, morphological, electron microscopical, statistical ones. It has been established that the effect of low dose irradiation results of first in reparation of lipid-protein granules from spermatozoon mirface and than caused destroying of its cytoskeleton in necrose-like manner. Afterwards the fragments of microfibrils were found in cultural medium. Apart from this, rupturing of acrosomes and plasma membrane were also discovered. In the dose range 10-50 Gy the raise in ion-transporting ATPase activities was accompanied by the increase in spermatozoa motility. Aptitude of epidydimal spermatozoa for post-irradiation recovery of sublethal and potentially lethal damages has been shown to be coupled with additional elevation of antioxidant enzymes activities, especially the catalase one. The survival of isolated oocytes has decreased in dependence on the irradiation dose in the dose-range 1-400 Gy. The oocytes were able to recover radiation lesion after fractionated and prolonged irradiation regimens. The peculiarities of M1 production from X-irradiated rat males and rat females were analyzed, the typical changes in phenotype of M1 offspring being. Product Description popup.authors popup.nrat_date 2020-04-02 Close