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Information × Registration Number 0307U009074, 0106U012515 , R & D reports Title To develop the method for increase of antitumor resistance of organism using dendritic cells loaded with tumor antigen in experiment and to determine indications for its use in complex therapy of cancer patients popup.stage_title Розробити схеми застосування дендритних клітин, навантажених пухлинним антигеном, в експерименті на моделі метастазуючої пухлини Head Khranovska N., Registration Date 25-12-2007 Organization Ukrainian Research Institute of Oncology and Radiology popup.description2 The aim of the study – Тo develop the method of immunotherapy using dendritic cells loaded with tumor antigen on the model of metastasizing tumor and to determine indications for its use for the improvement of antitumor resistance of organism of the patients with malignancies. It has been revealed that there is a direct dependence of antitumor effect of dendritic cells (DC) on a dose of tumor antigen (TA) used for their loading. In study of antimetastatic activity of DC such dependence was not observed. Dependence of antitumor effect of DC on their amount and ways of infusion is determined. It is proved that in animals with tumors their ability to migrate is significantly decreased independently of the way of their administration as compared with intact animals. The highest migration activity of DC to lymphoid organs occurs in intravenous way of administration, the lowest – in subcutaneous. Use of TA with increased immunogenic properties (lysate of tumor cells treated by lectines Bacillus subtilis B-7025) for loading of DC potentiates their antimetastatic effect. It has been detected that combination use of DC-vaccines with preparation “Ukrain” is significantly effective as for inhibition of development of metastases of melanoma B6 to lung of mice as compared with their separate use. Dependence of phenotypic properties of DC of monocytic origin on dose of TA for loading is studied. It has been found that “Ukrain” stimulates maturation of DC with maximal effect at concentration of 0,6 ?g/ml, which is a therapeutic one, in tissues of the patients after use of preparation. A protocol of DC-vaccine therapy in complex treatment of patients with ovarian, lung cancer and melanoma has been used. It has been proved that it is possible to obtain mature DC suitable for use as antitumor vaccines in patients with various forms of malignancies. Product Description popup.authors popup.nrat_date 2020-04-02 Close