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Information × Registration Number 0310U008310, 0106U004047 , R & D reports Title To develop modern diagnostics methods of viral infections of agricultural plants popup.stage_title Розробка молекулярно-біологічних методів діагностики ВЖКЯ (вірусу жовтої карликовості ячменю) на осногві ПЛР. Head Bojko Anatolij Leonidovich, Registration Date 23-12-2010 Organization Institute of agroecology and rural economics NAASU popup.description2 Developed methods of diagnosis of viral infections of crops on the basis of indirect ELISA.The method is based on using polyclonal rabbit anti-sera obtained before the next viral antigens: a bromus mosaic virus (BrMV), zucchini yellow mosaic virus (ZYMV), cucumber green mottle mosaic virus (CGMMV) and cucumber mosaic virus (CMV). Examined and sampled plant family Cucurbitaceae of two natural zones of Ukraine: mixed forest (Kiev region) and forest (Poltava region). Established that during the period from 2006 to 2010 in these agricultural lands CMV, ZYMV and Watermelon mosaic virus 2 (WMV 2) were circulated. Their circulation had a wavelike character, which is associated with the ways of their transmission. From vegetable agrocenoses were isolated the viruses as CMV, ZYMV, WMV2, and BrMV. It was shown that the basic properties of Ukrainian viral isolates did not differ substantially from those described in the literature, strains and isolates of these viruses. Highly purified viruses used as a positive control in indirect ELISA. Also, a group of authors published and implemented 5 methodical recommendations. Product Description popup.authors Бисов Андрій Сергійович Бойко Анатолій Леонідович Руднєва Тетяна Олександрівна popup.nrat_date 2020-04-02 Close