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Registration Number

0312U003427, 0109U002117 , R & D reports

Title

The investigation of mitochondria and plasma membrane ion channels role in vasospasm and myocardial dysfunction development

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Вивчення ролі калієвих каналів плазматичної мембрани гладеньком'язових клітин у розвитку артеріального вазоспазму і міокардіальної дисфункції різного ґенезу в умовах in vitro та in vivo

Head

Soloviev A.,

Registration Date

14-06-2012

Organization

State Establishment "Institute of Pharmacology and Toxicology National Academy of Medical Science of Ukraine"

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The decrease of the potassium transmembrane currents amplitude caused by 6 Gy ionizing radiation (IR) induced oxidative stress has been shown in rat aorta smooth muscle cells (SMC) . Currents through KATF-channels were without change. The total current amplitude was decreased probably due to inhibition of BKCa currents. Analysis of the genetic potential of KV and KATF channels showed overexpression of mRNA Kv 1.5. channels and Kir 6.2.- subunit KATF channels in irradiated animals. The mitochondrial pore (MP) blocker cyclosporine A (CsA) being added to the incubation medium has no effect on potassium currents in healthy animals and produced significantly increase after irradiation. It may indicate the involvement of MP in the development of SMC dysfunction in oxidative stress. Acetylcholine-induced vascular relaxation is not accompanied by changes in intracellular Ca2 + in endothelial cells of irradiated animals. In addition, transient component of relaxation was arisen, and the paxilline induced BKCa blockade has no effect on the aorta SMC relaxation. However, calcium sensitivity coefficient decreases in irradiated animals in the stable phase of ACh-induced relaxation. MP blockade restores a suppressed respiratory activity and energy production in mitochondria of irradiated cardiomyocytes. Reperfusion of isolated heart after subtotal ischemia causes inhibition of pump and contractile function, which was increased by CsA. However, CsA completely prevents the development of reperfusion arrhythmias.

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Іванова Ірина Володимирівна
Бойцова Людмила Василівна
Данова Ірина Владиславівна
Добреля Наталія Володимирівна
Зеленський Сергій Миколайович
Кізуб Ігор Володимирович
Мончак Ігор Леонідович
Паршиков Олександр Вікторович
Стрелков Євген Валентинович
Тишкін Сергій Михайлович
Хромов Олександр Станіславович

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2020-04-02