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Information × Registration Number 0215U004818, 0113U000460 , R & D reports Title Methodological approaches elaboration to the matter of biological preservation of tissue typing of cord blood samples providing as a basis for the creation of donor hematopoietic tissue cryobanks popup.stage_title Head Kalynychenko T.O., Доктор медичних наук Registration Date 04-12-2015 Organization Kiev Research Institute of Haematolojy and Blood Transfusion popup.description2 Research object: cord blood nuclear cells, cord blood red cell concentrate. Methods: сryobiological, morphological, biochemical, cultural, immunohematological, cytogenetic, molecular genetics. Conditions for securing the safety of human cord blood hematopoietic tissue have been examined. Methodical approaches to the creation of its histo-, AV0- and Rh- typed cryopreserved reserves have been analyzed. The feasibility of low concentration using of DMSO (5 %) by freezing in liquid nitrogen vapor has been shown for the hemopoietic tissue cord blood bank functioning. This is due to the possibility of exclusion of the preparatory phase before the recipient transfusion. The technology of freezing human cord / placental red blood cells at the temperature - 196 °C has been developed. This includes the using of the mixture of the cryoprotectants with different action mechanisms in the low total effective concentration (15 %) and simplified laundering stage after thawing. Medical and social effect. Medicine. Product Description popup.authors Андреєва С.В. Аношина М.Ю. Балан В.В. Гордієнко А.І. Дмитренко О.О. Калиниченко Т.О. Мінченко Ж.М. Павлюк Р.П. popup.nrat_date 2020-04-02 Close