Search academic texts

Information × Registration Number 0225U000889, (0122U000770) , R & D reports Title Determination of osteoconductive and osteoinductive properties of polymer-hydroxyapatite biodegradable scaffolds popup.stage_title Оцінка токсичності та біосумісності отриманих біополімері Head Oleshko Oleksandr M., Кандидат медичних наук Registration Date 21-01-2025 Organization Sumy State University popup.description1 The aim of the project is to establish general laws of the creation of three-dimensional polymer hydroxyapatite scaffolds in order to control resorption processes in biological conditions and biocompatibility of implants modified in composition and structure, as well as their antibacterial properties due to the inclusion of silver and zinc oxide nanoparticles. Objectives of the study: 1. Development of optimal concentrations of HA and PCL / PLA ratios, as well as a selection of modes (temperature, metric parameters of pores, and printing fibers) of 3D-print to create three-dimensional polymer-nanohydroxyapatite scaffolds. 2. Creation of three-dimensional polymer-nanohydroxyapatite scaffolds with the addition of different concentrations and shapes of silver and zinc oxide nanoparticles. 3. Determination of physicochemical, corrosion, structural and optical characteristics of the surface of implants obtained by different methods. 4. Determination of the degree of adhesion, proliferation, and metabolism of cells during cultivation on implants with a functionalized surface. 5. Generalization of research results in terms of the possibility of creating biodegradable orthopedic implants popup.description2 The following new scientific results were obtained in the course of the research work: 1. A mathematical analytical model based on computer simulation and a laboratory methodology with optimal PCL/PLA ratios and concentrations of nanohydroxyapatite and silver nanoparticles have been developed; 3D printing algorithm with mode selection (temperature, pore metric parameters and printing fibre) for the creation of three-dimensional polymer-nanohydroxyapatite biodegradable scaffolds. 2. It was found that the combination of PLA and PCL led to the formation of a porous not only the surface, but also the structure of scaffolds. An increase in the PCL content causes a decrease in stiffness and an increase in ductility of the material. The mechanical properties of the 85% PLA 15% PCL 15% HA and 75% PLA 25% PCL 10% HA samples are as similar as possible to those of trabecular bone tissue. 3. It was found that the combination of PLA and PCL led to the formation of a porous surface of scaffolds, i.e. an increase in the total area, which positively affects the intensity of cell adhesion. The enrichment of copolymers with hydroxyapatite nanoparticles (optimal concentration 12.5 %) has a stimulating effect on the adhesion and proliferation of osteoblasts. The enrichment of scaffold with silver nanoparticles leads to a significant decrease in the activity of biofilm formation of microbial cells on the surface of samples as a result of the antimicrobial effect of nanoparticles and causes a gradual decrease in the number of CFU of microbial cells due to the release of silver ions in the process of material degradation. Product Description popup.authors Yevdokymenko Vladyslav Yu. Berladir Khrystyna V. Hlushchenko Victoriia V. Holub Dmytro O. Deineka Yaroslav V. Dryhval Bohdan О. Dudko Yuliia S. Kidymenko Andrii O. Lukianenko Maksym V. Malysheva Tetiana V. Oleshko Oleksandr M. Oleshko Tetiana B. Ostroha Ruslan O. Sikora Kateryna O. Serdiukov Yehor V. Yanishevska Vladyslava Yu. popup.nrat_date 2025-01-21 Close