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Information × Registration Number 0208U000235, 0105U002648 , R & D reports Title The significance of persistent viral infection in pathogenesis of chronic lymphoproliferative diseases and cytopenias of different origin in distant period after Chornobyl catastrophe popup.stage_title Head Chumak Anatoliy Andreevich, Доктор медичних наук Registration Date 22-01-2008 Organization Research Centre for Radiation Medicine popup.description2 The object of study: 336 patients, among them are 188 B-cell chronic lymphoid leukemia (B-CLL) patients (47 patients with ionizing radiation (IR) exposure and 141 control patients), 7 patients with other chronic lymphoproliferative diseases (5 - clean-up workers of Chernobyl NPP accident), 16 patients with myelodysplastic syndrome, MDS (8 - suffered due to Chernobyl NPP accident), 19 multiple myeloma patients, 64 patients with benign hematological disorders (34 - suffered due to Chernobyl NPP accident), 42 clean-up workers without hematological diseases. The aim of study is to determinate the significance of TORCH infections, hepatitis B and C viruses in development of chronic lymphoproliferative diseases, myelodysplastic syndrome and cytopenias of undetermined significance in patients suffered due to Chernobyl NPP accident. Methods of study are polymerase chain reaction (PCR) with direct sequencing of PCR products, immunoenzyme assay, immunocytochemical detection of antigen's expression, clinical,statistical methods. This is the first investigation of IGHV rearrangement in CLL patients who have been exposed to ionizing radiation due to the Chernobyl NPP accident. Clean-up workers of 2nd quarter of 1986 (n=19) were picked out as separate group with the highest number of unmutated cases (94.4%), increased usage of IGHV1-69 (33.3%) and IGHV3-21 (16.7%) genes, high frequency of secondary solid tumors (6 cases). These preliminary data suggest that CLL in the most suffered contingent due to Chernobyl NPP accident might have some specific features. High level of contamination CLL patients with persistence infections and increased frequency of IgVH3 genes usage in cytomegalovirus (CMV) and Toxoplasma gondii infection were revealed that suggested about possible stimulation of certain B-lymphocyte clones under influence of viral and bacterial antigens. In group of clean-up workers without hematological diseases the association between the infection of virus of hepatitis C (HCV), CMV and Т. gondii and the tendency to lowering of erythrocytes and hemoglobin content in peripheral blood were found. High level of HCV infection and signs of CMV reactivation in bone marrow cells were found in MDS patients, that might deepen of depression of hematopoiesis. Taking into account likely contribution of persistence infections in CLL pathogenesis (activation of certain B-lymphocyte's clones as precursors of disease development) and their inhibitory influence on hematopoiesis, it is necessary to determinate, establish of activity and in proper time treat these infections for prophylactics and during development of disease. Product Description popup.authors popup.nrat_date 2020-04-02 Close