Search academic texts

Information × Registration Number 0208U002282, 0103U000075 , R & D reports Title A study on the model objects of the expected therapeutic effects is from introduction of rekombinantnikh molecules popup.stage_title Head Kordium Vitaliy Arnoldovich, Registration Date 24-01-2008 Organization Institute of molecular biology & genetics popup.description2 Report on NIR: 2p. 184 references, 101 рисунків, 14 tables. A research object is рекомбінантні molecules. A purpose of work is a study on the model objects of the expected therapeutic effects from introduction of рекомбінантних molecules. Research methods are a technique of рекомбінантних molecules, ?ioiioa?iaioiee analysis, cellular technologies, histological analysis. Work was conducted on four directions. After the first direction technology of genetic correction of saccharine diabetes of 1th type and atherosclerosis was developed by introduction of рекомбінантних molecules with having a special purpose genes, that gene therapy. The row of molecular vectorial constructions is constructed with the fullsize genes of препроінсуліну and аполіпопротеїну A1 of man and the effective unviral system of transgenesis in vitro and in vivo is developed. For standardization of receipt of preparations of vectorial DNK devices which allowed to provide the producibility of indexes of efficiency of трансфекції are created. On the model of Stc-?ndukovanogo of diabetes for mise dependence of therapeutic action is set on the dose of preparation of рекомбінантної DNK and certain its optimum amount which results in complete indemnification of diabetes for animals. The models of Stc-?ndukovanogo are developed ?inoe?icaea?iiai diabetes for pigs to the breed of Landras and in the mine of piggy-wiggies of breed the «Vietnamese hop-o'-my-thumb». On these models of long duration therapeutic effects (returning of pathosis of animals to the norm) were certain from introduction of рекомбінантних DNK with the gene of insulin of man. The results of regression of diabetes for model animals after non-permanent gene therapy are confirmed патоморфологическими researches of organs, цитологіичними researches of maintenance of hepatin, in cages livers which rotined normalization of processes of carbohydrate exchange, and by biochemical researches of the system of oxide of nitrogen (NO) in fabrics of heart, buds and liver of experimental animals. On the model of experimental atherosclerosis for crawls the minimum dose of рекомбінантної DNK, which contains the gene of аполіпопротеїну A1 of man introduction of which by a direct injection in a liver under control BRIDLES, results in indemnification of typical for atherosclerosis of defeats fabrics and vessels of model animals, is certain, and it is rotined that introduction of this dose of preparation with artificial гіперхолестеринемією blocks development of atherosclerosis crawls on a background a quiescent load a cholesterol during the 3х months of experiment. To second direction of work of department preparation of рекомбінантного growth of man hormone was described on authenticity, degree of cleanness and biological activity, the results of which answered the requirements of European Pharmacopoeia in relation to medicinal preparations. It is rotined that, in obedience to the got descriptions, the probed preparation of рекомбінантного growth hormone can be entered any model objects, and also man.After the third direction with the use of ZT-PLR of technology it is cloned in the cages of E.coli gene of LIF of man and his expression is got. Biological activity is rotined рекомбінантного squirrel. A genetic construction is created for expression of LIF in еукаріотичних cages. The last direction is related to research of influence of the combined introduction of genes interleukin-2 and interleukin-12 mise in composition recombinant constructions on a seroresponse which is induced immunization of DNK the created model vaccines. It is rotined that genes interleukin-2 and interleukin-12 потенцируют протективну activity of gene vaccines. Product Description popup.authors popup.nrat_date 2020-04-02 Close