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Information × Registration Number 0208U005613, 0103U005876 , R & D reports Title The investigation of molecular basis of functional and biological activities of polymers (lipopolysaccharides, enzymes) of microbial cell popup.stage_title Head Varbanets Lyudmila Dmitrievna, Registration Date 07-07-2008 Organization Zabolotny Institute of Microbiology and Virology Ukrainian NAS popup.description2 At first,the OPS structure of investigated R.aquatilis strain were established.It was identified 5 types of OPS structure,which were unique.The structure of OPS were represented by linear or branched hetero- or homopolysaccharides,which include mono-,three-,hexa-(or hepta-)saccharides repeating units.OPS of some strains tested included more than one type of oligosaccharide,which were different by their structure.On the basis of O-antigenicity of LPS,strains investigated were devided in to three serogroups.LPS of all investigated strains are pyrogenic and toxic.Chemical modifications of LPS by N,O-deacylation and dephosphorylation lead to the complete loss of their toxicity and pyrogenicity.As result of screening among 364 stains of microorganisms of different taxonomy groups it were found two producers of collagenase and keratinase-Streptomyces sp.1349 and Streptomyces sp.1382,respectively.Three collagenases and two keratinases were obtained from culture liquids of strain-producers.It was found two collagenases are metalloproteases,and other collagenases and keratinases are serine proteases.Physical and chemical properties,specificity of action of purified enzymes were investigated.It has been shown that preparations of enzymes are differ in their molecular weights,composition of amino acids,optimal conditions of their action,sensitivity for cations of metalls and anions.Collagenases are characterized by wide specificity to different protein substrates, while keratinases are able to hydrolyze only native keratin of wool or feathers.Also,one of keratinase has high fibrinolytic activity.It has been shown that collagenases of Streptomyces sp.1349 have no serological affinity for enzyme for Clostridium histolyticum ("Merk") Product Description popup.authors popup.nrat_date 2020-04-02 Close