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Information × Registration Number 0209U005022, 0108U006623 , R & D reports Title The development of cryotechnology for cell and tissue therapy popup.stage_title Head Gryschenko Valentyn I., Registration Date 12-01-2009 Organization Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine popup.description2 Steady growth of human fibroblasts and stromal stem marrow elements in a culture was obtained. Their ability was shown for adgession and proliferation after cryopreservation under 7% DMSO and fetal serum. It was shown that the presence of nano particles in cryopreservation solution resulted in stabilization of overcooling value, inconsiderably influenced precrystallization process during the system thawing and does not influence on temperature of the verification. Introduction of allogeneic native and cryopreserved mesenchymal stem cells derived from bone marrow and human fibroblast culture to the area of thick-layer knee joint cartilage defect results in cartilagelike tissue formation in defect area to compare with the control group of animals. Product Description popup.authors popup.nrat_date 2020-04-02 Close