Registration Number
0209U010556,
0107U008259
, R & D reports
Title
Variable biotechnology usage for the correction of morphofunctional state of myocardium in experimental cardiomyopathy condition.
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Head
Zaporozhan Valeriy,
Registration Date
15-12-2009
Organization
Odessa state medical university
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Two methods of progenitor cells cultivation were discovered: long protocol and short protocol. Bone marrow was obtained from the femoral bones of ICR mice, put into salt solution, afterwards was centrifuged. The sediment was centrifuged in Percoll gradient (with the densities 1,065 - 1, 073), then centrifugation in DMEM medium with 10 % nutritive medium FBS cells were shared on the 10 mattresses NUNC with 15 ml of DMEM/F12, in 1:1 ratio, 10 % FBS, penicillin (0,2 / ml), streptomycin-sulfate 5x10-6 g/ml. Medium was changed every third day during the cultivation. Short cultivation continued 7 days, long cultivation - 21 day. After the end of cultivation monolayer of the cells was taken off with the tripsine - EDTA solution. Comparative analysis showed absence of differences in cell culture quality after both long and short protocols of cultivation.
Product Description
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Запорожан Валерій Миколайович
Перепелюк Миколай Миколайович
Пихтєєв Дмитро Михайлович
Холодкова Олена Леонідівна
Щербатюк Аліна Леонідівна
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2020-04-02
