Registration Number
0209U010557,
0107U008260
, R & D reports
Title
Creation of the methods of mesenchimal stem cells cultivation with the row of growth factors usage for the correction of pathological states in experiment.
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Head
Zaporozhan Valeriy,
Registration Date
15-12-2009
Organization
Odessa state medical university
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The methods of cultivation of bone marrow of mice and introduction of obtained MSCs into mice with adriblastine-induced toxic hepatitis were worked. Cultivation was carried out on two charts: with addition in the nourishing medium of fibroblast growth factor (FGF) or factor of endothelial cell growth (EGF). After 21 day of cultivation cells in an amount of 450000 per animal were entered in a tail vein to 30 mice with experimental toxic hepatitis. In 10 days was found out the substantial positive influence of MSCs on the morphological state of hepatic tissue regardless of the applied chart of cultivation. However, the EGF application resulted in more clear structural regeneration of the beam system of hepatic lobule. Reliable differences in quality of the cellular material got in different ways are not exposed.
Product Description
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Запорожан Валерій Миколайович
Кордюм Віталій Арнольдович
Перепелюк Миколай Миколайович
Пихтєєв Дмитро Михайлович
Холодкова Олена Леонідівна
Щербатюк Аліна Леонідівна
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2020-04-02
