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Information × Registration Number 0210U000974, 0107U010968 , R & D reports Title Creation of hybrid nanoparticles as supersensitive fluorescent sensors of new generation, modification of their surface and development of colloidal gold biospecific nanoparticles for immunocemical assay popup.stage_title Head Komisarenko Serhiy Vasylyovych, Demchenko Olexandr Petrovich, Registration Date 26-04-2010 Organization A.V.Palladin Institute of Biochemistry of National Academy of Sciences popup.description2 Developed algorithm of analysis of dye fluorescence spectra in basal and excited states allows to determine separately parameters of hydratation and polarity. It was shown perspectives of using the developed approach for study of Parkinson's disease and others protein folding diseases. Assay system prototype and method for qualitative valuation of immunochemical reactions with using of universal biospecific sonde - conjugate of colloidal gold nanoparticles with recombinant protein A fragment for immunochromatografic detection of antibodies from different animals and human were developed. For this E.coli, producing recombinant protein A fragment, was created, optimal conditions for colloidal gold nanoparticles synthesis and for its conjugating with protein A fragment were selected. Densitometry approach to semi-qualitative evaluation of immunochromatographic test results was developed. Parameters that can influence on accuracy of immunochromatografic test evaluation were studied. Product Description popup.authors Горчев Василь Федорович Кабернюк Андрій Аркадійович Колибо Денис Володимирович Короткевич Наталія Валеріївна Лабинцев Андрій Юрієвич Назаренко Володимир Іванович Олійник Олена Сергіївна Редчук Тарас Анатолійович Романюк Світлана Іванівна Тарасенко Алла Сергіївна popup.nrat_date 2020-04-02 Close