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Information × Registration Number 0210U003444, 0108U000759 , R & D reports Title Natural enemies of oak pests as a factor regulating their densities: ecological-genetic approach popup.stage_title Head Ivashov Anatoliy Vasil'evich, Registration Date 09-02-2010 Organization Taurida National V.I. Vernadsky University popup.description2 Object of investigation: gypsy moth, oak leaf roller and their natural enemies - nucleopolyhidrosis virus and parasitoids. Eim of the investigation: foundation of the principially new ecological and genetic approaches to the pest control with application of DNA-insecticides and natural evolutionary-genetic processes intending decreasing pest damage potention. Method of investigation: field and laboratory experiments. First determined the RAPD-PCR markers of the five drugs tested different geographical origin of the virus. Processing of gypsy moth larvae with short DNA fragments of viral gene inhibitor of apoptosis LdnVgp140 leads to an increase in the death of the larvae of the pest by 30% compared with the control. This effect can be used for the production of bio-insecticides. Obtained RAPD-PCR, allozyme and phenotypic markers of oak, green oak leaf roller and four of its parasites, allowing to describe the genetic preference in the interaction of species in the food chain: Oak-Tortrix-parasites. Up to 49% of the total fitness of parasites have on the interaction of factors "fen / genotype of the parasite" - "fen leafroller" and up to 28% - on the interaction of factors "fen parasite" - "genotype of oak. Thus, the genetic variability of the green oak leaf roller and its host plants affects the fitness components of its parasites. Key words: pubescent oak, oak leaf roller moth, gypsy moth, parasitoids of oak leaf roller moth, gypsy moth nucleopolyhidrosis virus, allozymes, DNA-analysis, PCR, RAPD-markers. Product Description popup.authors Івашов Анатолій Васильович Оберемок Володимир Володимирович Разумейко Володимир Миколайович Сімчук Андрій Павлович popup.nrat_date 2020-04-02 Close