Registration Number
0210U003839,
0107U004944
, R & D reports
Title
Development of combined gene therapy for type 1 diabetes and vascular pathologies
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Head
Kordium Vitalii Arnoldovich, Доктор біологічних наук
Registration Date
17-03-2010
Organization
Institute of Molecular Biology and Genetics
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Current work which was conducted according to plan touches development of the approach to simultaneous application of two target genes of human preproinsulin and apolipoprotein A1 for treating of multifactorial diseases on the models of Type 1 diabetes and concomitant diseases. Transfection experiments on cultivated mammalian cells were carried out. The expression efficiency of engineered gene cassettes and functional activity of designed vectors has been tested.The stable transformad mammalian cell lines expressing both human target proteins have been received. Gene therapy in vivo experiments in mice and rats with experimental diabetes were performed and the therapeutic effect of recombinant DNA containing target genes has been shown. It has been shown that consequently simultaneous expression of preproinsulin and apolipoprotein A1 in liver cells decrease of fasting hyperglycemia was attended by the reduction of NO stable methabolites to normal, that is evidence of considerable therapeutic effect of engineered construction.
Product Description
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Іродов Д.М.
Гулько Т.П.
Моргунов П.В.
Похоленко Я.О.
Римар С.Ю.
Рубан Т.П.
Сухорада О.М.
Топорова О.К.
Шпильова С.П.
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2020-04-02
