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Information × Registration Number 0210U004060, 0107U005245 , R & D reports Title Portative analyzer based on amperometric enzyme-biosensors for the quality control of beverages in wine-making. Isolution of preparative quantities of the stabilized enzymes. popup.stage_title Head Sibirny Andrei, Registration Date 08-02-2010 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 Object of the work: alcohol oxidase from methylotrophic yeast Hansenula polymorpha, glycerol oxidase from mould fungus Bоtrytis allii; wines. The aim of the work: to develop the experimental sample of compact analyser on the base of amperometric enzymatic biosensors for analysis of wines and control their quality. The aim of the work, part 3 (ICB, NAS of Ukraine): isolation of preparative quantities of enzymes: alcohol oxidase from Hansenula polymorpha and glycerol oxidase from Bоtrytis allii. The aim for the 2009 year stage (part 3): to compare the data of biosensoric and referent methods for determination of ethanol, lactate, glycerol and glucose in wines and wine's products. Methods of investigations: microbial, chemical, physico-chemical, analytical, enzymatic. Results of the work: optimal conditions for microbial strains overproducing alcohol oxidase and glycerol oxidase were investigated; the cells with high yield of the enzymes were obtained: preparative isolation, purification, stabilization were made and physico-chemical characteristics of enzymes (alcohol oxidase and glycerol oxidase) as bioselective elements of biosensors were investigated; the determination of glucose, ethanol and glycerol content in model solutions and real wine's samples by standard methods was done; the new enzymatic photometric method for lactate analysis based on flavocytochrome b2 of the yeast H. polymorpha was developed and tested on the real samples of wines; the comparison of the data obtained by using different analytical methods (enzymatic and biosensoric) for ethanol, lactate, glycerol and glucose assay in wines was done. Product Description popup.authors Гайда Г.З. Гончар М.В. Дмитрук К. В. Клепач Г.М. popup.nrat_date 2020-04-02 Close