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Information × Registration Number 0210U007471, 0105U005343 , R & D reports Title Study of the role of molecular chaperones in the development of apoptosis in cardiomyocytes popup.stage_title Head Sidorik L.L., Registration Date 30-12-2010 Organization Institute of Molecular Biology and Genetics popup.description2 Heart failure is a leading cause of death in developed countries, claiming more lives than other major illnesses. Many data specify the important pathogenic role of the dysregulation of anti-stress signaling in myocardium in DCM The balance between renaturation of proteins after stress and their degradation is very important for the development or preventing the autoimmunity. HSPs, together with other components, seem to be the key regulators for multiple signaling pathways involved in growth and development, apoptosis, autoimmunity induction and heart failure progression. Interactions between HSPs and accessory proteins are important for cell survival and any disturbances might induce alterations in cardiac cells physiology and cardiomyocyte death, resulting in the development of the heart failure. The progression of heart failure is associated with the decrease of the anti-apoptotic potential of the myocardium, as manifested by mitochondrial defects leading both to the rise of autoimmune processes, and to the start of apoptotic destruction of cardiomyocytes with the subsequent fibrosis of cardiac muscle (typical for DCM) During the last decade a great number of data has been obtained, confirming a potential cardioprotective role of the basic cytoplasmic chaperons . However, the role of mitochondrial premature chaperon Hsp60 in human heart failure progression, and mechanisms of Hsp60 involving in apoptosis regulation are poorly understood. We are firstly observed positive correlation in Hsp60 and p70S6K expression at heart failure progression at acute phase (myocarditis) and chronic phase (dilated cardiomyopathy) as well. Such correlation have been observed in dynamics - on the experimental model of inducible autoimmune injury of mice myocardia like human DCM. The study of cellular localization of such antigens in diseasesd myocardia revealed the differences in re-localization of studied proteins: Hsp60 was located mainly in cardiomyocytes although the p70S6K shown the extensive re-localization to cardiac stroma at heart failure progression. We are firstly observed a different immunoreactivity of Hsp60 and p70S6K in sera of patients affected by DCM and ICM what could be a real base in creation of diagnostic tool for cardiomyopathies of different genesis. The in vivo complex between Hsp60 and protein Bax was identified in normal myocardia in comparison with DCM-affected ones, and complex between Hsp60 and p70S6K was observed in human myocardia. We proposed a working hypothesis concerning the possible role of molecular chaperons in the regulation of stress-induced signal pathways of cardiomyocytes. Product Description popup.authors І.В. Крупська Бобик В.І. Капустян Л.М. О.Г.Вігонтіна О.М.Федоркова Рожко О.Т. Яковенко Л.Ф. popup.nrat_date 2020-04-02 Close