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Information × Registration Number 0211U004519, 0110U006127 , R & D reports Title Development of the system for growth inhibition of brain tumors on the basis of nanoconjugates of antisens-oligonucleotides and antibody, specific for oncoproteins, with natural bioplymers popup.stage_title Head Kavsan Vadym Moiseevitch, Registration Date 04-02-2011 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 The aim of the project is development and creation of the system for the suppression of the growth of brain glial tumors by nanoconjugates with new effective targeted antitumor drugs. It will be created Polycefin - nanoconjugate of natural polymer poly-beta-malic acid (poly- -malic acid, PMLA) from microorganism Physarum polycephalum, with antisense CHI3L1 mRNA oligonucleotides or antibodies against the CHI3L1 protein, and other functional modules. Polycefin will be used for the treatment of glial tumors for antisense inhibition of protein target. Basic modules, necessary for further synthesis of Polycefin, were obtained as a resut of the fullfilment of first project stage. Procedures of PMLA activation and bonding of functional modules to polymeric matrix were worked out. Recombinant CHI3L1 protein was synthesized in prokaryotic system and purified by affine chromatography on Ni-NTA-agarose. Native CHI3L1 protein was isolated from conditioned medium of human osteosarcoma cell line MG-63 and purified by affine chromatography on heparine-sepharose. Product Description popup.authors Єршов Антон Вікторович Балинська Олена Вікторівна Дмитренко Володимир Володимирович Дубей Лариса Василівна Костенко Олександр Миколайович popup.nrat_date 2020-04-02 Close