Search academic texts

Information × Registration Number 0211U005784, 0107U008260 , R & D reports Title Creation of the methods of mesenchimal stem cells cultivation with the row of growth factors usage for the correction of pathological states in experiment. popup.stage_title Head Zaporozhan Valeriy, Registration Date 14-12-2011 Organization Odessa State Medical Universitu popup.description2 It was revealed after quantitative and qualitative assessment of mice bone marrow and adipose tissue cell cultures that there is no significant differences between qualitative and quantitative composition of cell cultures derived from different sources. But there is an advantage in a method of adipose tissue cells growing, which is in a simpler way of obtaining the material and in technical performance of cultivation. Cultivation for 21 days according to various protocols showed that accordingly to the first protocol with the addition of cytokine cocktail containing NGF and retinoic acid, and the second protocol using HGF, TNF- and TNF- a significant differences in the quality of cellular material were not found. Throughout the period of number of living cells and their morphological status was estimated. With the help of immunocytochemistry method cells were identified that were differentiated in hepatogenic and neurogenic directions using the hepatocyte or neurocyte nuclear factor respectively, that were applied on a layer of obtained cells, and after the usual cycle immunocytochemistry with morphometric method using Avtandylov net to estimate a number of received differentiated cells. It was confirmed that cells that were cultured according to the first protocol differentiated into neurogenic direction, and the cells that are cultured with the second protocol - in hepatogenic one. Product Description popup.authors Білявський Олександр Іванович Запорожан Валерій Миколайович Котельник Олена Леніанівна Кулєшова Олена Анатоліївна Нескоромна Наталія Владиславівна Ославська Тетяна Михайлівна Попов Олександр Георгієвич Холодкова Олена Леонідівна popup.nrat_date 2020-04-02 Close