Registration Number
0211U006244,
0107U009690
, R & D reports
Title
Mechanisms of enhancing sensitivity of leukemic cells to chemotherapeutic drugs using natural modifiers of apoptosis
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Head
Philchenkov Olexy Olexiyovych,
Registration Date
02-03-2011
Organization
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology
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Jurkat/А4 cells obtained upon exposure of Jurkat T-cell human leukemia cells to anti-Fas monoclonal antibody and possessing multiple drug resistance phenotype have been shown to be resistance to X-ray radiation and apoptosis induction by natural polyphenols quercetin and resveratrol. In Jurkat/А4 cells, the content of proapoptotic proteins Bad and BNip-1 was reduced, while the content of antiapoptotic proteins CAS and XIAP was elevated as compared with that in the initial Jurkat cell line. The irradiation of Jurkat/А4 cells followed by quercetin exposure resulted in the enhancement of the apoptotic effect of ionizing radiation. In cells of patients with B-cell chronic lymphoblastic leukemia, preincubation with quercetin for 1-3 days resulted in significant sensitization to induction of apoptosis by DNA-topoisomerase II inhibitor vepeside. In Jurkat/А4 cells resistant to cytotoxic chemotherapeutic substances the sensitivity to apoptosis induction is reduced independent of apoptosis inducers used (cytotoxic drugs, polyphenols, ionizing radiation).
Product Description
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Завелевич Михайло Петрович
Квілинська Анастасія Ігоревна
Лупан Валентина Григорівна
Фільченков Олексій Олексійович
Шостак Катерина Олександрівна
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2020-04-02
