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Information × Registration Number 0211U008683, 0111U008338 , R & D reports Title Impact studying of cultured multipotent mesenchymal stromal cells of various origin on the various etiology trophic ulcer healing popup.stage_title Head Ostapchenko Liudmila I., Registration Date 26-08-2011 Organization Taras Shevchenko Kiev university popup.description2 It was resulted, in vitro MSCs cultures isolated from different tissues of C57BL/6 and CBA mice (dermis, thymus stroma, adipose tissue, bone marrow stroma) were obtained. The tests for osteo- and adipogenesis differentiations of cultured MSCs were also carried out. The acute wound was accomplished in the experimental group of CBA mice with induced by high-dose streptozotocin injection (240 mg/kg) hyperglycemia (glucose level in blood was 218-244 mg/dL). The living dermal equivalent consisting of polymerized collagen gel and cultured adipose syngeneic MSCs (1 x 106 cells/ml) was implanted on modeled diabetic ulcers. In the control group of mice was observed complete healing of the wound skin defect to the 25th day after the acute injury. In both cases, in experimental group of animals with hyperglycemia to the 25th days the non-healing of modeled chronic diabetic ulcers was observed, but a variant with a dermal equivalent implantation resulted to the wound area decreasing up to 5% (in comparing with 5th day value), without the implantation - up to 26% (in comparing with 5th day value). Product Description popup.authors Зубов Д. О. Остапченко Л. І. popup.nrat_date 2020-04-02 Close