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Information × Registration Number 0212U000794, 0107U000536 , R & D reports Title Studying the state of genetic apparatus and its functional manifestation in stem hemopoietic cells with the aim to improve the treatment methods for diseases of autoimmune genesis. popup.stage_title Head Goltsev Anatoly Mykolayovuch, Registration Date 03-04-2012 Organization Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine popup.description2 The research objects were CBA/H and (CBA/HxC57B1) F1 20-weeks' mice of 24-26 g and fetal liver cells (FLCs) of CBA/H mice of the 14th (FLCs-14) and 18 th (FLCs-18) gestation days. The research aim was to improve the treatment methods for autoimmune diseases (AIDs) based on the study of genetic apparatus state and its functional manifestation in hematopoietic stem (HSCs) and mesenchymal stem cells (MSCs). In the research there were used modern methods of the studies such as: cytofluorimetric, immune enzyme and PCR-analysis method. There was developed the experimental model of one type of autoimmune diseases, especially an acute type, s graft-versus-host disease (GVHD). There was found the reduced content of hematopoietic stem (HSCs) and mesenchymal stem (MSCs) cells, as well as T-regulatory (T-reg) cells against bone marrow hypoplasia, developing as a result of immune disease as GVHD. Taking into account the possible use for treating GVHD the preparations of fetal origin, in the work there were assessed the phenotype characteristics and state of genetic apparatus of HSCs and MSCs of fetal liver of different gestation terms prior to and after cryopreservation. It has been established the correcting effect of FLCs as for the cells of stem compartment of the recipients with GVHD, as well as Treg cells with suppressive activity and reduced the pathology severity. The highest correcting effect was inherent to cryopreserved FLCs of late gestation terms, which in greater extent stimulated the expression of genes, responsible for hemopoietic activity of HSCs and immune suppressive of MSCs. The studies are or priority character and enable not only the widening of our notion on the mechanisms of development of autoimmune conflicts on cell and molecular levels, but and substantiate the expediency of the directed increase in the efficiency of treatment of one of the forms of autoimmune diseases, GVHD with co-transplantation of histoincompatible bone marrow with FLCs. Product Description popup.authors Бабенко Наталья Миколаївна Гаєвська Юлія Олександрівна Дубрава Тетяна Георгіївна Луценко Олена Дмитрівна Останков Максим Вадимович Останкова Людмила Василівна Чуб Наталья Нестерівна popup.nrat_date 2020-04-02 Close