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Information × Registration Number 0212U001430, 0110U000232 , R & D reports Title Molecular genetic mechanisms of radiation-associated oncohematological pathology forming in victims of the Chernobyl NPP accident. popup.stage_title Head Klymenko Sergiy Viktorovich, Доктор медичних наук Registration Date 03-02-2012 Organization Research Centre for Radiation Medicine popup.description2 Object: myelodisplastic syndrome/acute myeloid leukemia, polycythemia vera, essential thrombocythemia and myelofibrosis in persons exposed to ionizing radiation due to the Chernobyl accident and patients without radiation history. Purpose: to determine the molecular genetic mechanisms of oncohematological pathology forming in persons exposed to ionizing radiation due to the Chernobyl accident. Methods: clinical, molecular-genetics, cells culture, immunologic, and statistics. The specificity and sensitivity of testing of JAK2 V617F presence was accessed for the use as a marker of tumor disorders of myeloid origin in patients with radiation associated and spontaneous pathology. We have used immunoferment analyzer Humareader (Germany), thermocycler Gene-Amp PCR 2700 (Singapore), microscope "Sedival" and "Standard 25" Carl Zeiss, Jena (Germany). Study subjects included 218 patients who were previously diagnosed as MDS/GML, PV, ET, PMF, among them 125 patients who was exposed to ionizing radiation from the Chernobyl accident, made a basic group. The opposing group was formed by 117 people with spontaneous pathology. The control group entered 74 patients with symptomatic erytrocytosis. Results and novelty. Despite of clinical futures of MPD associated with radiation exposure, which are in variability range of diagnostic criteria of disease. The JAK2 V617F mutation was detected by multiplexed allele-specific polymerase chain reaction. The frequency of JAK2V617F mutation in patients with spontaneous and associated with radiation exposure PV was 95,16% and 94,74%, ET - 50% and 50%, PMF - 68,18% and 38,46%, MDS/GML - 0% and 0% accordingly. For patients with radiation associated OMF is determined tendency, on border statistical significance (r=0,08) to more rare prevalence of mutation of V617 F the gene of Jak2. The specificity and sensitivity of the use Jak2 V617 F assay for diagnostic of spontaneous and radiation associated PV is 100% and 91,67%, 95,35% and 94,73% accordingly, PMF - 100% and 100%, 68,18% and 38,5% accordingly. The specificity and sensitivity of the test for ET radiation associated ET did not differ and was 50% and 100% accordingly. The positive and negative prognostic value of the assay of mutation status the gene of Jak2 V617F for diagnostics spontaneous and associated with radiation exposure PV, ET, PMF testifies about of expediency of inclusion this assay in diagnostic verification algorithm. The detection of mutation of Jak2 V617F for establishing the diagnosis of spontaneous and radiation associated MDS/GML is characterized an insufficient sensitivity and specificity, and low positive and negative prognostic significance. The result of the cultivation of hematopoietic stem cell of peripheral blood in the environment on the basis of methylcellulose is demonstrated correlation of amount of clusters and colonies for patients with PV is 6:1 and 4:1, after MDS - 3:1 and 4:1, after PMF - 5:1 and 5:1 in an environment with autoserum and in an environment with exogenous erythropoietin accordingly, that can be used for diagnostics of MPD. Product Description popup.authors Балан В. В. Дмитренко І.В. Клименко С. В. Костюкевич О.М. Любарець Т.Ф. Міщенюк О.Ю. Прокопенко І.М. Федоренко В.Г. Шолойко В.В. popup.nrat_date 2020-04-02 Close