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Information × Registration Number 0212U001965, 0111U005988 , R & D reports Title Macromolecules and their complexes in realization of genetic information popup.stage_title Head El'skaya Anna Velentynivna, Доктор біологічних наук Registration Date 04-09-2012 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 As a result of the second phase of the research project "Macromolecules and their complexes in the realization of genetic information" project participants studied heterogeneous structural properties of macromolecular complexes and components associated with the performance of their biological functions, and perspectives for further research in this direction. Thus, studies of biologically important molecular systems such as model protein-nucleic complexes sugar residues of DNA and RNA, as well as canonical and modified 2'-dexoxyribonucleosides. Particular attention is paid to the nature of non-covalent interactions responsible for the stability and functioning of the systems. Hypothetically formed rare tautomers that may arise under the influence of DNA-binding proteins of replysoma will not have any effect on spontaneous mutations in DNA, due to the low reverse reaction barrier that allows nucleotide bases quickly return to the canonical form. For hydrogen bonds OH ... O, OH ... N, NH ... O and OH ... C in DNA components correlated well between the shift of the corresponding characteristic stretching vibrations at low frequencies and the value of the electron density ?cp at a critical point (3, -1) that allows to set the ratio between ? and energy for each connection. It was determined that no conformational barriers are there for incorporation of 2 ', 3'- didezoxy- 2', 3'-diderybonucleosides in the DNA double helix in the A and B forms. Less pronounced biological activity d4U comparison with 2 ', 3'- didezoxy -2', 3'-didehydrothymidyne (d4T), probably related to the presence of bulk methyl group in the 5th position of d4T basis. The data on the structural and functional organization of the active center of bacterial prolyl-tRNA synthetase in complex with alanyl-tRNKPro and mechanism of catalysis of deacylation are obtained. The most important functional element of this mechanism is the 2'-OH A76 alanyl-tRNKPro, which forms a hydrogen bond with the carbonyl group of alanine residue, and acts as the general acid in the substrate-assisted catalysis. The amino acid residues of the active center is involved in coordinating substrates and auxiliary water molecule that carries out proton transfer to O3 'A76. Thus, tRNA plays an active role in the process of posttransfer editing that provides amino acid specificity ProRS. First shown that subunit complex eEF1V may be in tumor tissues in individual state, that exist independently of the complex. First time the nuclear localization of subunit eEF1B?.The relative nespoluchnist Cancer dependent changes of mRNA levels and subunit eEF1B, encoded mRNA data are shown. Existence subodynts eEF1B separately from the complex in tumor tissues indicates their ability to perform certain non-canonical functions in carcinogenesis. The existence of endogenous splaysovoyi shape kinase ribosomal protein S6 - S6K1/?15 is identified and confirmed. Found that its expression inhibits S6K1 and mTOR and consequently affects cell proliferation. A new protein complex S6K1 / 2 with adapter protein TDRD7 is identified. It is found that TDRD7 is a substrate for S6K1 / 2. Phosphorylation sites of S6K1/2-depending TDRD7 are identified. Defined genetic and epigenetic damage WNT7A gene in pale renal carcinomas. It was found correlation between genetic / epigenetic changes and gene expression WNT7A. Additionally, it was shown that the restoration of gene expression WNT7A a depressant effect on the formation of colonies and cell proliferation renal carcinoma. It was found a discrepancy between the level of mature and immature miRNA, suggesting the participation of not only the transcriptional regulatory mechanisms of miRNAs but post transcriptional, especially in the acute phase reoxygenation whereas prolonged process, which is the postinfarction remodeling, mainly characterized transcriptional regulation level miRNAs. Hipocalcin is a key mediator of many cellular functions, including NMDA receptor-dependent long-term depression and slow posthyperpolarization. As a result, his study showed that hipokaltsyn may be site-specific mediator of high dynamic range, which allows strict modulation of their targets. Product Description popup.authors Б.С.Негруцький В.Є.Досенко В.І.Кашуба, д.б.н. В.В.Філоненко Г.В.Єльська Г.Д.Яремчук Д.М.Говорун Л.Г.Горб М.А.Тукало П.В.Білан popup.nrat_date 2020-04-02 Close