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Information × Registration Number 0212U002178, 0109U001563 , R & D reports Title The investigation of ionizing radiation modificational effect on chromosome stability in somatic cells of a human by means of testing mutagenic exposure of bleomycin popup.stage_title Head Pilinskaya Maria, Доктор медичних наук Registration Date 27-01-2012 Organization Research Centre for Radiation Medicine popup.description2 Subject: human blood lymphocytes. Objective: To investigate the possibility of modifying the genetically determined susceptibility of human somatic cells' chromosomes to the testing mutagenic exposure in vitro because of previous effects of ionizing radiation in vivo. Methods: Cytogenetic. Results: Four surveillance groups had been formed - conditionally healthy donors (comparison group 1); Chernobyl liquidators (professional group 1), persons suffering from lung cancer who denied conscious contact with mutagenic factors (comparison group 2); Chernobyl liquidators with realized oncopathology (professional group 2). Voluntary cytogenetic examination of individuals from these groups had been performed; the background and induced (using the modified by us test "G2-bleomycin sensitivity assay") frequency of chromosome aberrations in peripheral blood lymphocytes had been established. It had been found that in all the groups mean background frequency of chromosome aberrations did not exceed the upper limit of population control, but only in two occupational groups unstable and / or stable cytogenetic markers of radiation exposure had been observed with different rate. Wide interindividual variation of cytogenetic effect induced by bleomycin and lack of positive correlation between the background and induced frequencies of chromosomal aberrations had been found in all examined groups. The addition to the mean background frequency of chromosome aberrations (above spontaneous cytogenetic effect) as well as percentage of persons with hidden chromosome instability (HChI) hypersensitive to bleomycin exposure were maximal in professional group 2. The results confirmed the possibility of radiation-induced modification of genetically determined susceptibility of human somatic cells' chromosomes to testing mutagenic bleomycin exposure and feasibility of using test "G2-bleomycin sensitivity assay" under cytogenetic examination of irradiated contingents to identify HChI as one of markers of predisposition to cancer. Product Description popup.authors Єрємеева М.Н. Дибська О.Б. Дибський С.С. Степанова Л.С. Сушко В.О. Сушко Л.І. popup.nrat_date 2020-04-02 Close