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Information × Registration Number 0212U002183, 0109U001568 , R & D reports Title Estimation of chronic lymphocytic leukemia risks among the contingents suffered due to Chornobyl catastrophe on the basis of investigation of gene coding the protein of reparation polymorphism popup.stage_title Head Chumak Anatoliy Andreevich, Доктор медичних наук Registration Date 27-01-2012 Organization Research Centre for Radiation Medicine popup.description2 The aim of study - to reveale an association between polymorphisms of some DNA repair genes and risk of chronic lymphocytic leukemia in sufferers of Chornobyl NPP catastrophe for early detection of risk grous of patients. The object of study - 198 chronic lymphocytic leukemia patients and 219 persons without oncohematological disorders.The methods of study: the investigation of DNA repair gene's polymorphisms using polymerase chain reaction and restriction of products, clinical, statistical. The main results: the frequencies of dominant and polymorphic alleles of DNA repair genes (XRCC1, XRCC3, XPD) and their combinations in CLL patients and controls who a residents of Ukraine were established. It was found that the distribution of abovementioned polymorphisms in controls did not differ regarding of radiation anamnesis and corresponded to others froups of Caucasians. Modifying role of DNA repair genes in CLL development under influence ionizing radiation (IR) was found: CLL risk in homozygotes for dominant allele of XPD, sufferers of Chornobyl NPP catastrophe, was diminished (OR = 0.37; 95% CI 0.1815-0.7572; р=0.005) expecially in combination with homozygous for dominant allele of XRCC1 gene (OR=0.28; 95% CI 0.085-0.935; р=0.03). On the other hand, CLL risk in homozygotes for polymorphic allele of XPD was increased: OR = 2.69 (95% CI 1.3207-5.5103, р=0.005). Some peculiarities of CLL were found depend on DNA repair gene's polymorphisms: increasing frequency of Richter transformation in patients with genotype Gln399Gln of XRCC1 after therapy; protective influence of genotype Gln751Gln of XPD. Increased number of basal cell cancer was found in IR-exposed carriers of polymorphic allele of XPD in comparison with non IR-exposed patients (p=0.009).The increased frequncy of chronic hepatitis (p=0.001), chronic gastritis (p=0.001) and goiters (p0.036) was found in homozygotes of Gln399Gln of XRCC1 in control group of IR-exposed patients. The influence of Gln399Gln of XRCC1 genotypes on these diseases was revealed under presence of persistance infection of hepatitis C viruses). Genotype Gln751Gln of XPD had protective influence on development of chronic somatic lung diseases in sufferers of Chornobyl NPP catastrophe(OR = 0.1875; 95% CI 0.0388-0.9024; р=0.023). The study of DNA repair gene's polymorphism may be helpful for prognosis of risk of CLL and some somatic diseases under IR exposure. Product Description popup.authors Абраменко Ірина Вікторівна Білоус Надія Іванівна Дягіль Ірина Сергіївна Костін Олексій Володимирович Плескач Гліб Вадимович popup.nrat_date 2020-04-02 Close