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Information × Registration Number 0212U002286, 0109U000619 , R & D reports Title Development of conception of epigenetic mechanisms of aging. Investigation of epigenetic pat-terns formation mechanics in process of organism aging popup.stage_title Head Bozhkov A.I., Registration Date 03-02-2012 Organization Kharkov National University named after V.N. Karazin popup.description2 Object of study - the epigenetic patterns, age dependence of characterization of epigenotype. Studies were performed on male rats of Wistar aged 3 and 21 months. Divided into groups, which were kept on different diets. Some groups were injected intraperitoneally three times at a dose of copper sulfate, 0.23 mg / g liver after 48 hours, studies were also carried out in cell culture microalgae Dunaliella viridis Teod. of the different ages. It was held definition of the chronological and replicative aging. Purpose - development of epigenetic mechanisms of aging concept and age dependence of epigenotype notion. Research Methods - biochemical (membrane-bound enzyme activity was determined glucose-6-phosphatase activity of glutathione reductase, glutathione transferase, isocitrate dehydrogenase, NADH-cytochrome c reductase and glutathione peroxidase Se-dependent), and pyrene fluorescence intensity associated with the membranes of the endoplasmic reticulum in the system in vitro. It was found that both young and old animals in the first cycle of losing the same amount of weight. In the future two weeks body weight was restored. When you cycle, young animals have already lost 34%, and the old - 25%. Further feeding of these animals within a month provided the increase in body weight by 71% and 16% of the young old, the third cycle was accompanied by the subject to the same conditions of weight loss in young a 7% increase compared to the first cycle and only 9% in old, t . e. by 17% compared to the first cycle. Further feeding will not provide full recovery of animal weight. It is shown that life expectancy is determined trained in the early stages of ontogenesis epigenotype. Experimentally demonstrated the presence of reversible and irreversible age-epigenotype. Proved the ability to maintain long-lived experiment potentially epigenotype in cell culture and animal models (calorie restricted diet). By irreversible epigenetic changes include processes of carbonylation of proteins and structural changes at the level of cell membranes Product Description popup.authors Асадова М.К. Длубовська В.Л. Клімова О.М. Кургузова Н.І. Малєєв В.А. Наглов О.В. Шаповалов С.О. popup.nrat_date 2020-04-02 Close