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Information × Registration Number 0212U004077, 0108U010328 , R & D reports Title In vitro and in vivo study of signaling mechanisms of induction of apoptosis depending on types of inducer and target cells popup.stage_title Head Stoyka Rostyslav, Registration Date 26-01-2012 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 New mechanisms of glycocalyx changes during apoptosis have been grounded experimentally. It was shown that there are two main types of sub-cellular membrane vesicles which appear during cell death and differ considerably by the ways of formation of desialated glycoepitopes. The vesicles that are formed from the endoplasmic reticulum of the apoptotic cells, contain unprocessed mannoside glycoproteins, while the vesicles that are detached from the plasma membrane of those cells, are modified by the membrane associated sialidase and contain chromatin components (DNA and histones), but do not contain the components of nuclear membrane. Earlier, we found that human macrophages could discriminate between these two types of the apoptotic vesicles. The obtained results are important for better understanding of the mechanisms of cell clearance at autoimmune and oncological diseases. It was found that blood serum of systemic lupus erymatosus patients, similar to blood serum of multiple myeloma patients, contains the catalytically active antibodies (abzymes) that possess sialidase activity. The detected abzymes can detach moieties of sialic acids from the carbohydrate chains of glycoproteins, as well as from the gangliosides. These data suggest participation of sialil-hydrolyzing abzymes in the processes of clearance of apoptotic and necrotic cells at some oncohematological and autoimmune diseases. It was revealed that at terminal stage of growth of murine ascitic NK/Ly lymphoma, there is a massive formation of sub-cellular microvesicles in the ascitic fluid by means of their detachment from the surface of these apoptotic cells. The immune antiserum with the titer 1:32 of agglutination of tumor cells, was obtained against the microvesicles isolated by the differential centrifugation. From that antiserum, the antibodies were purified, and they are under study for their antitumor activity in mice bearing NK/Ly lymphoma. Product Description popup.authors Антонюк В.О. Білий Р.О. Бойко Н.М. Кіт Ю.Я. Кащак Н.І. Луцик М.Д. Панчук Р.Р. Стойка Р. С. Філяк Є.З. popup.nrat_date 2020-04-02 Close