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Information × Registration Number 0212U004847, 0110U002207 , R & D reports Title To study features of the biological response of an organism in dynamics of interferon therapy at skin melanoma patients and to determine immunological criteria of efficiency of the combined treatment popup.stage_title Head Filchakov F.V., Registration Date 06-02-2012 Organization Ukrainian Research Institute of Oncology and Radiology popup.description2 Object of research: immunocompetent cells, аutoantibodies. The purpose - To determine features of shifts in immune system of patients skin melanoma under influence interferon therapy for forecasting efficiency of the combined treatment. Methods of research - immunological, flow cytometry, a method of cell culture, statistical. The equipment: centrifuges ОPN-3; a microscope; flowing cytometer FACScan; the automatic sampleres; termostates; the СО2-incubator. Clinical and immunological investigation of 27 patients with histological confirmed diagnosis skin melanoma with metastasises in regional lymph nodes of a ІІІВ-ІІІС stage in dynamics of the combined treatment with application of different interferon therapy regimes has been studied. It is established, that carrying out of induction IFN-alpha2b therapy irrespective of the regimen of application (up to or after surgical treatment) is accompanied by development of the leukocytopenia directly after its ending; in case of its application in neoаdjuvant regimen - quantity of leukocytes and lymphocytes in peripheral blood of patients is restored after operation. Induction IFN-alpha2b therapy in neoаdjuvant regimen promotes restoration after surgical treatment of quantity T-lymphocytes in peripheral blood up to norm, but does not liquidate increased Тrеg contents. The similar rate of interferon therapy in аdjuvant regimen does not restore of decreased quantity T-lymphocytes, but promotes reduction of Тrеg level in circulation. Induction IFN-alpha2b therapy both in neoаdjuvant, and in аdjuvant regimen, promotes reduction in peripheral blood of patients of increased contents of activated lymphocytes (HLA-DR+ and CD95+). In case of application of induction and supporting rates IFN-alpha2b therapy in аdjuvant regimen increased relative quantity CD4+25+-lymphocytes up to a level at practically healthy individuals (PHІ) also is reduced. As against it at reception by patients in аdjuvant regimen only a supporting rate - relative quantity CD4+25+-lymphocytes stably high during all term of supervision, than is possible to explain preservation on increased level relative contents Тrеg. Induction IFN-alpha2b therapy in neoаdjuvant regimen results in decrease of cytotoxic lymphocytes activity, and in аdjuvant - to restoration cytotoxic lymphocytes activity up to level PHІ. Induction IFN-alpha2b therapy in neoаdjuvant regimen (not in аdjuvant) promotes restoration of the answer lymphocytes on mitogen in vitro up to norm. After supporting IFN-alpha2b therapy at patients of basic group ability of lymphocytes is restored to produce of MIF. Irrespective of regimen of application induction IFN-alpha2b therapy (neoаdjuvant or аdjuvant) dynamics of changes thyroid autoantibodies and ТSH in serum is unidirectional: level ТSH remains within the limits of norm, and concentration TPO-Ab and ТG-Аb decreases after 3 months supporting IFN-alpha2b therapy. SKIN MELANOMA, INTERFERON THERAPY, LYMPHOCYTES, IMMUNOPHENOTYPE, CYTOTOXIC ACTIVITY, PROLIFERATION LYMPHOCYTES Product Description popup.authors Ільченко Ю.М. Кукушкіна С.М. Льон Г.Д. Шуміліна К.С. popup.nrat_date 2020-04-02 Close